caa a22 4500 397590555 CHVBK 20180308164937.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/jac/37.5.901 doi (NATIONALLICENCE)oxford-10.1093/jac/37.5.901 Choice of a routine method for detecting methicillin-resistance in staphylococci [Elektronische Daten] [F. Wallet, M. Roussel-Delvallez, R. J. Courcol] Four methods were compared for their abilities to detect methicillin-resistance of Staphylococcus strains using mecA gene PCR analysis in 6 h as the gold standard. 57 Staphylococcus aureus and 100 coagulase negative staphylococci (CNS) were evaluated by the oxacillin disc diffusion method (Kirby-Bauer), the automated API (ATB-plus) system (bioMérieux, La Balme les Grottes, France) in 24 h, the rapid BBL Crystal MRSA ID system (Becton Dickinson, Cockeysville, Md.), the oxillin MICs using the NCCLS agar dilution method in 24 h, and the mecA gene PCR analysis. For S. aureus, the correlation was excellent between the BBL Crystal MRSA ID system and mecA gene PCR analysis (positive predictive value=100%; negative predictive value=97%) and oxacillin MIC (positive predictive value=96%; negative predictive value=96%). The correlation between BBL Crystal MRSA ID and mecA gene PCR was not reliable for CNS (negative predictive value=68%). For CNS, the slower routine susceptibility methods to identify intrinsic methicillin-resistance were better: API ATB Staph has a positive predictive value=94% and a negative predictive value=82%, and the disc diffusion test has a positive predictive value=95% and a negative predictive value=74%. However, BBL Crystal MRSA ID was as reliable as some of the other methods tested for CNS after 6 h incubation when the inoculum was increased: positive predictive value=94% and a negative predictive value=77%. These results emphasize that genotypic detection of methicillin-resistance will undoubtedly become important to detect rapidly methicillin-resistance, especially for CNS. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Wallet F. Laboratoire de Bactériologie-Virologie-Hygiène Höpital A. Calmette, CHRU de Lille, 59037 Lille cedex, France aut Roussel-Delvallez M. Laboratoire de Bactériologie-Virologie-Hygiène Höpital A. Calmette, CHRU de Lille, 59037 Lille cedex, France aut Courcol R. J. Laboratoire de Bactériologie-Virologie-Hygiène Höpital A. Calmette, CHRU de Lille, 59037 Lille cedex, France aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/5(1996-05), 901-909 0305-7453 37:5<901 1996 37 jac https://doi.org/10.1093/jac/37.5.901 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.5.901 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wallet F. Laboratoire de Bactériologie-Virologie-Hygiène Höpital A. Calmette, CHRU de Lille, 59037 Lille cedex, France aut NATIONALLICENCE 700 1- Roussel-Delvallez M. Laboratoire de Bactériologie-Virologie-Hygiène Höpital A. Calmette, CHRU de Lille, 59037 Lille cedex, France aut NATIONALLICENCE 700 1- Courcol R. J. Laboratoire de Bactériologie-Virologie-Hygiène Höpital A. Calmette, CHRU de Lille, 59037 Lille cedex, France aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/5(1996-05), 901-909 0305-7453 37:5<901 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford