caa a22 4500 397590636 CHVBK 20180308164937.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/jac/37.3.465 doi (NATIONALLICENCE)oxford-10.1093/jac/37.3.465 Rapid high performance liquid chromatographic assay for antifungal agents in human sera [Elektronische Daten] [T. K. C. Ng, R. C. Y. Chan, F. A. B. Adeyemi-Doro, S. W. Cheung, A. F. B. Cheng] Serum concentration of seven antifungal agents, amphotericin B, 5-flucytosine, ketoconazole, fluconazole, itraconazole, miconazole and econazole were assayed using a single step sample preparation and an isocratic High Performance Liquid Chromatography (HPLC) procedure based on three mobile phases of similar components. Our method was simple, flexible and rapid, the assays being completed within half an hour. The method showed high reproducibility, good sensitivity with detection limits of 0.078 to 0.625 mg/L except for miconazole and econazole, and high recovery rates of 86-105%. Out of 24 therapeutic agents tested only aztreonam and trimethoprim were found to interfere with the assay of 5-flucytosine and fluconazole respectively, using this protocol. HPLC assay should be useful in the clinical laboratory for monitoring patients on antifungal therapy. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Ng T. K. C. Department of Microbiology, Prince of Wales Hospital Shatin New Territories, Hong Kong aut Chan R. C. Y. Department of Microbiology, The Chinese University of Hong Kong Shatin New Territories, Hong Kong aut Adeyemi-Doro F. A. B. Department of Microbiology, The Chinese University of Hong Kong Shatin New Territories, Hong Kong aut Cheung S. W. Department of Microbiology, Prince of Wales Hospital Shatin New Territories, Hong Kong aut Cheng A. F. B. Department of Microbiology, The Chinese University of Hong Kong Shatin New Territories, Hong Kong aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 465-472 0305-7453 37:3<465 1996 37 jac https://doi.org/10.1093/jac/37.3.465 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.3.465 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ng T. K. C. Department of Microbiology, Prince of Wales Hospital Shatin New Territories, Hong Kong aut NATIONALLICENCE 700 1- Chan R. C. Y. Department of Microbiology, The Chinese University of Hong Kong Shatin New Territories, Hong Kong aut NATIONALLICENCE 700 1- Adeyemi-Doro F. A. B. Department of Microbiology, The Chinese University of Hong Kong Shatin New Territories, Hong Kong aut NATIONALLICENCE 700 1- Cheung S. W. Department of Microbiology, Prince of Wales Hospital Shatin New Territories, Hong Kong aut NATIONALLICENCE 700 1- Cheng A. F. B. Department of Microbiology, The Chinese University of Hong Kong Shatin New Territories, Hong Kong aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 465-472 0305-7453 37:3<465 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford