caa a22 4500 397590652 CHVBK 20180308164937.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/jac/38.2.205 doi (NATIONALLICENCE)oxford-10.1093/jac/38.2.205 CAT III chloramphenicol resistance in Pasteurella haemolytica and Pasteurella multocida isolated from calves [Elektronische Daten] [Cécile Vassort-Bruneau, Marie-Claude Lesage-Descauses, Jean-Louis Martel, Jean-Pierre Lafont, Elisabeth Chaslus-Dancla] Chloramphenicol, which had been used extensively for antimicrobial veterinary therapy, was prohibited in Europe in 1994. Soon after it became available, resistance to this drug was detected, generally conferred by plasmids encoding inactivating enzymes, the chloramphenicol acetyltransferases (CAT), in Gram-negative as well as in Gram-positive bacteria. In the last few years, resistance to antibiotics emerged in Pasteurella strains from braeding herds and this evolution was followed by a national surveillance network. Chloramphenicol-resistance was more recently detected in multiresistant strains. We studied 25 strains of Pasteurella, selected for their resistance to chloramphenicol. Production of a CAT was demonstrated in all these strains. PCR amplification indicated that the CAT produced was of type III for 23 of them. In these strains, chloramphenicol-resistance was mediated by plasmids of about 5.1 kb. Southern blots on restriction fragments suggested a high degree of homology between these 5.1 kb plasmids. In the two other strains, production of a CAT type I was demonstrated, and the corresponding genes were either shown on a plasmid of 17 or 5.5 kb. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Vassort-Bruneau Cécile Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly 37380, Monnaie aut Lesage-Descauses Marie-Claude Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly 37380, Monnaie aut Martel Jean-Louis Centre National d' Etudes Vétérinaires et Alimentaires BP 70330, 69342, Lyon, France aut Lafont Jean-Pierre Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly 37380, Monnaie aut Chaslus-Dancla Elisabeth Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly 37380, Monnaie aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/2(1996-08), 205-213 0305-7453 38:2<205 1996 38 jac https://doi.org/10.1093/jac/38.2.205 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.2.205 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vassort-Bruneau Cécile Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly 37380, Monnaie aut NATIONALLICENCE 700 1- Lesage-Descauses Marie-Claude Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly 37380, Monnaie aut NATIONALLICENCE 700 1- Martel Jean-Louis Centre National d' Etudes Vétérinaires et Alimentaires BP 70330, 69342, Lyon, France aut NATIONALLICENCE 700 1- Lafont Jean-Pierre Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly 37380, Monnaie aut NATIONALLICENCE 700 1- Chaslus-Dancla Elisabeth Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly 37380, Monnaie aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/2(1996-08), 205-213 0305-7453 38:2<205 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford