caa a22 4500 397590857 CHVBK 20180308164938.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/jac/37.4.737 doi (NATIONALLICENCE)oxford-10.1093/jac/37.4.737 The incidence and epidemiology of β-lactam resistance in Haemophilus influenzae [Elektronische Daten] [P. A. James, D. A. Lewis, J. Z. Jordens, J. Cribb, S. J. Dawson, S. A. Murray] Consecutive isolates of Haemophilus influenzae were collected by the Public Health Laboratory, Bath between 1 June 1992 and 31 May 1993. Of 379 apparently distinct isolates, 216 originated from the respiratory tract, 102 from eyes and 61 from other sites. The minimum inhibitory concentrations of amoxycillin, amoxycillin/clavulanate, cefaclor, cefuroxime, cefotaxime and cefpodoxime were determined for each isolate. Forty strains (10.6%) were β-lactamase producers. MIC50 and MIC90 values and the range of MICs were determined for all isolates. The overall resistance rates were: amoxycillin (MIC>1.0 mg/L), 22.7%; amoxycillin/clavulanate (MIC>1.0 mg/L), 14.8%; cefuroxime (MIC>1.0mg/L), 18.5%, (MIC>4.0mg/L), 5.5%; cefaclor (MIC>8 mg/L), 15.6%; cefpodoxime (MIC>1.0mg/L), 0.3%; cefotaxime (MIC>1.0 mg/L), 0%. Twenty non-β-lactamase producing but β-lactam resistant strains (cefuroxime MIC>4.0 mg/L) were matched with 20 susceptible strains on the basis of patient age, sex, and specimen type. The strains were characterised by outer-membrane protein (OMP), random amplified polymorphic DNA (RAPD) and ribotyping patterns. Eleven of the 20 resistant strains were indistinguishable by the methods used, suggesting spread of a single β-lactam resistant, non-β-lactamase producing clone. The distribution of resistant strains within the local community was plotted geographically. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence James P. A. Public Health Laboratory, Royal United Hospital (NHS Trust) Bath; UK aut Lewis D. A. Public Health Laboratory, Royal United Hospital (NHS Trust) Bath; UK aut Jordens J. Z. PHLS Haemophilus Reference Laboratory, John Radcliffe Hospital Oxford, UK aut Cribb J. Public Health Laboratory, Royal United Hospital (NHS Trust) Bath; UK aut Dawson S. J. Public Health Laboratory, Royal United Hospital (NHS Trust) Bath; UK aut Murray S. A. Public Health Laboratory, Royal United Hospital (NHS Trust) Bath; UK aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/4(1996-04), 737-746 0305-7453 37:4<737 1996 37 jac https://doi.org/10.1093/jac/37.4.737 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.4.737 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- James P. A. Public Health Laboratory, Royal United Hospital (NHS Trust) Bath; UK aut NATIONALLICENCE 700 1- Lewis D. A. Public Health Laboratory, Royal United Hospital (NHS Trust) Bath; UK aut NATIONALLICENCE 700 1- Jordens J. Z. PHLS Haemophilus Reference Laboratory, John Radcliffe Hospital Oxford, UK aut NATIONALLICENCE 700 1- Cribb J. Public Health Laboratory, Royal United Hospital (NHS Trust) Bath; UK aut NATIONALLICENCE 700 1- Dawson S. J. Public Health Laboratory, Royal United Hospital (NHS Trust) Bath; UK aut NATIONALLICENCE 700 1- Murray S. A. Public Health Laboratory, Royal United Hospital (NHS Trust) Bath; UK aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/4(1996-04), 737-746 0305-7453 37:4<737 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford