caa a22 4500 397590911 CHVBK 20180308164938.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/jac/37.6.1077 doi (NATIONALLICENCE)oxford-10.1093/jac/37.6.1077 Antimicrobial activity of cecropins [Elektronische Daten] [Alison J. Moore, Wayne D. Beazley, Michael C. Bibby, Deirdre A. Devine] The lytic peptides, cecropins, were originally isolated from the haemolymph of the giant silk moth, Hyalophora cecropia and possess antibacterial and anticancer activity in vitro. This study investigated the antimicrobial activity of these peptides against human pathogens using standardised assay techniques, and the activity of cecropin B on outer and inner bacterial membranes. From a panel of 15 organisms, Gram-negative bacteria were generally more sensitive to cecropins than Gram-positive organisms, especially the lipopolysaccharide defective mutant, Escherichia coli BUE55. Cecropins B and P1 shared similar MIC values whereas Shiva-1, a cecropin B analogue, was less active. Through combination studies with hydrophobic antibiotics and electron microscopy, cecropin B was shown to disrupt the bacterial outer membrane. Protoplasts of Staphylococcus aureus and Staphylococcus epidermidis were resistant to cecropin B, suggesting that the cytoplasmic membranes of Gram-positive organisms were inherently more resistant to the peptide. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Moore Alison J. Department of Biomedical Sciences, University of Bradford West Yorkshire, BD7 1DP, UK aut Beazley Wayne D. Department of Biomedical Sciences, University of Bradford West Yorkshire, BD7 1DP, UK aut Bibby Michael C. Clinical Oncology Unit, University of Bradford West Yorkshire, BD7 1DP, UK aut Devine Deirdre A. Department of Biomedical Sciences, University of Bradford West Yorkshire, BD7 1DP, UK aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/6(1996-06), 1077-1089 0305-7453 37:6<1077 1996 37 jac https://doi.org/10.1093/jac/37.6.1077 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.6.1077 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moore Alison J. Department of Biomedical Sciences, University of Bradford West Yorkshire, BD7 1DP, UK aut NATIONALLICENCE 700 1- Beazley Wayne D. Department of Biomedical Sciences, University of Bradford West Yorkshire, BD7 1DP, UK aut NATIONALLICENCE 700 1- Bibby Michael C. Clinical Oncology Unit, University of Bradford West Yorkshire, BD7 1DP, UK aut NATIONALLICENCE 700 1- Devine Deirdre A. Department of Biomedical Sciences, University of Bradford West Yorkshire, BD7 1DP, UK aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/6(1996-06), 1077-1089 0305-7453 37:6<1077 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford