caa a22 4500 397590970 CHVBK 20180308164938.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/jac/38.5.799 doi (NATIONALLICENCE)oxford-10.1093/jac/38.5.799 Effect of RO 23-9424, cefotaxime and fleroxacin on functions of human polymorphonuclear cells and cytokine production by human monocytes [Elektronische Daten] [G. Matera, M. C. Berlinghieri, F. Foti, G. S. Barreca, A. Focà] The way in which an antibiotic interacts with host defences could influence the clinical outcome of many infectious diseases. The impact of RO 23-9424, a novel dual-action and extended-spectrum antibiotic, was studied on several functions of human polymorphonuclear neutrophils (PMNs). A significant (P < 0.05) increase of the superoxide (O2−) released by phorbol-myristate acetate (PMA) -stimulated PMN (10-100 mg/L) can be observed in the RO 23-9424 pre-treated cells. RO 23-9424, particularly at low dosages, showed an interesting but not statistically significant effect on PMN phagocytosis. Higher dosages of RO 23-9424 (50-200 mg/L) and fleroxacin (20-200 mg/L) significantly reduced PMN chemotaxis. Cytokine production by human monocytes were also evaluated after incubation with the antibiotic (100-200 mg/L) in both basal conditions and in response to endotoxin (lipopolysaccharide, LPS). In the LPS-treated cells, RO 23-9424 (100 mg/L) significantly (P < 0.05) enhanced the tumour necrosis factor-α (TNF-α) levels, compared with LPS controls after 4 h of incubation. RO 23-9424 (200 mg/L) was able to reduce in a dose-dependent way LPS-induced interleukin-1β (IL-1β) after 4 and 24 h of incubation. Interleukin-8 (IL-8) release was not significantly changed by RO 23-9424. Cefotaxime (200 mg/L) significantly (P < 0.05) increased the basal levels of IL-1β and reduced basal IL-8 concentration after 24 h of incubation. The lower concentration of cefotaxime reduced the LPS-stimulated IL-8 levels. Fleroxacin (100 mg/L) enhanced basal levels of IL-8. The potentiated PMN phagocytosis, the significantly enhanced O2− release by PMA-stimulated PMN and the dimetric changes of TNF-α and IL-1β appeared peculiar for RO 23-9424 and may have useful therapeutical indications. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Matera G. Department of Microbiological, Neurological and Orthopaedic Sciences, Chair of Microbiology, University of Reggio Calabria, Cantanzaro, Italy Berlinghieri M. C. Department of Microbiological, Neurological and Orthopaedic Sciences, Chair of Microbiology, University of Reggio Calabria, Cantanzaro, Italy aut Foti F. Department of Microbiological, Neurological and Orthopaedic Sciences, Chair of Microbiology, University of Reggio Calabria, Cantanzaro, Italy aut Barreca G. S. Department of Microbiological, Neurological and Orthopaedic Sciences, Chair of Microbiology, University of Reggio Calabria, Cantanzaro, Italy aut Focà A. Department of Microbiological, Neurological and Orthopaedic Sciences, Chair of Microbiology, University of Reggio Calabria, Cantanzaro, Italy aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/5(1996-11), 799-807 0305-7453 38:5<799 1996 38 jac https://doi.org/10.1093/jac/38.5.799 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.5.799 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matera G. Department of Microbiological, Neurological and Orthopaedic Sciences, Chair of Microbiology, University of Reggio Calabria, Cantanzaro, Italy NATIONALLICENCE 700 1- Berlinghieri M. C. Department of Microbiological, Neurological and Orthopaedic Sciences, Chair of Microbiology, University of Reggio Calabria, Cantanzaro, Italy aut NATIONALLICENCE 700 1- Foti F. Department of Microbiological, Neurological and Orthopaedic Sciences, Chair of Microbiology, University of Reggio Calabria, Cantanzaro, Italy aut NATIONALLICENCE 700 1- Barreca G. S. Department of Microbiological, Neurological and Orthopaedic Sciences, Chair of Microbiology, University of Reggio Calabria, Cantanzaro, Italy aut NATIONALLICENCE 700 1- Focà A. Department of Microbiological, Neurological and Orthopaedic Sciences, Chair of Microbiology, University of Reggio Calabria, Cantanzaro, Italy aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/5(1996-11), 799-807 0305-7453 38:5<799 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford