caa a22 4500 397591063 CHVBK 20180308164938.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/jac/37.6.1121 doi (NATIONALLICENCE)oxford-10.1093/jac/37.6.1121 Rifampicin loading of vascular grafts [Elektronische Daten] [P. Malassiney, O. Goëau-Brissonnière, M. Coggia, J.-C. Pechère] Antibiotic-bound arterial prostheses may contribute to the management of prosthetic infections. The in-vitro absorption and release of rifampicin was measured in four different polyester arterial prostheses, including unsealed VP 1200, gelatin-sealed Gelsoft, collagen-impregnated Hemashield and gelatin-sealed Unigraft. Gelsoft grafts bound more rifampicin than unsealed VP 1200. Rifampicin concentrations with the gelatin sealed to Gelsoft grafts reached a threshold when the rifampicin concentrations of the soaking solution were 10 mg/mL or more. Rifampicin adsorption plateaued after 15 min of soaking VP 1200, and peaked after 25 min of soaking Gelsoft. pH variations did not significantly influence antibiotic binding. Prosthesis-bound rifampicin concentrations decreased rapidly after soaking, but a significant portion of the antibiotic remained associated with the material after 7 days. Release was slower with Gelsoft than with VP 1200 during the first 3 h, but after 24 h, the amounts of rifampicin released were similar in the two materials. Other experiments were performed in dogs receiving sealed grafts as infrarenal aortic bypass after soaking the material in a 1 mg/mL solution of rifampicin in normal saline. After 3 days of implantation, the amount of rifampicin in explanted grafts was higher in Gelsoft and Hemashield than in Unigraft. This study confirms that rifampicin bonding to prosthetic material occurs and is enhanced by sealed collagen or gelatin. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Malassiney P. Department of Genetics and Microbiology, Faculty of Medicine Geneva, Switzerland aut Goëau-Brissonnière O. Division of Vascular Surgery, Hôpital Ambroise Paré Boulogne-Billancourt, France aut Coggia M. Division of Vascular Surgery, Hôpital Ambroise Paré Boulogne-Billancourt, France aut Pechère J.-C Department of Genetics and Microbiology, Faculty of Medicine Geneva, Switzerland aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/6(1996-06), 1121-1129 0305-7453 37:6<1121 1996 37 jac https://doi.org/10.1093/jac/37.6.1121 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.6.1121 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Malassiney P. Department of Genetics and Microbiology, Faculty of Medicine Geneva, Switzerland aut NATIONALLICENCE 700 1- Goëau-Brissonnière O. Division of Vascular Surgery, Hôpital Ambroise Paré Boulogne-Billancourt, France aut NATIONALLICENCE 700 1- Coggia M. Division of Vascular Surgery, Hôpital Ambroise Paré Boulogne-Billancourt, France aut NATIONALLICENCE 700 1- Pechère J.-C Department of Genetics and Microbiology, Faculty of Medicine Geneva, Switzerland aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/6(1996-06), 1121-1129 0305-7453 37:6<1121 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford