caa a22 4500 397591144 CHVBK 20180308164939.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/jac/38.3.387 doi (NATIONALLICENCE)oxford-10.1093/jac/38.3.387 Bactericidal effect of plaunotol, a cytoprotective antiulcer agent, against Helicobacter pylori [Elektronische Daten] [Tetsufumi Koga, Harumi Kawada, Yukio Utsui, Haruki Domon, Chika Ishii, Hiroshi Yasuda] In order to investigate the bactericidal effect of plaunotol, an oily antiulcer agent, against Helicobacter pylori, comparative studies were conducted using its derivatives, M-4, M-5, and M-6, whose hydrophobicity decreased in the order of plaunotol > M-6 > M-5 > M-4 by log P determination. Plaunotol rapidly reduced the viability of H. pylori in vitro, and cell death was associated with cell lysis. In addition, plaunotol showed eightfold stronger bactericidal activity against H. pylori than M-6 and M-5, while the compound with the lowest hydrophobicity, M-4, showed no bactericidal activity. The bactericidal activities of plaunotol and its derivatives were related to the hydrophobicity of these compounds. To investigate a possible interaction between these compounds and the cell membrane of H. pylori, their effects on liposomal membranes prepared from phosphatidylethanolamine and cardiolipin, which are known to be present in the membrane of H. pylori, were determined by detection of glucose release from the liposomes. Plaunotol showed eight-fold higher activity than M-6 and M-5, while M-4 showed no activity. The effects of plaunotol and its derivatives on liposomal membrane were therefore related to their bactericidal activities. In addition, it was confirmed that the bactericidal effect of plaunotol against H. pylori was neutralized by the liposomal membrane, and that plaunotol led to an increase in permeability of the membrane, as evidenced by measurement of the leakage of 260 nm absorbing-material from H. pylori. These results suggest that the bactericidal effect of plaunotol against H. pylori is due to the interaction between this compound and the bacterial cell membrane. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Koga Tetsufumi Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut Kawada Harumi Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut Utsui Yukio Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut Domon Haruki Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut Ishii Chika Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut Yasuda Hiroshi Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/3(1996-09), 387-397 0305-7453 38:3<387 1996 38 jac https://doi.org/10.1093/jac/38.3.387 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.3.387 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koga Tetsufumi Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut NATIONALLICENCE 700 1- Kawada Harumi Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut NATIONALLICENCE 700 1- Utsui Yukio Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut NATIONALLICENCE 700 1- Domon Haruki Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut NATIONALLICENCE 700 1- Ishii Chika Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut NATIONALLICENCE 700 1- Yasuda Hiroshi Biological Research Laboratories, Sankyo Co., Ltd, 2-58 Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/3(1996-09), 387-397 0305-7453 38:3<387 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford