caa a22 4500 397591233 CHVBK 20180308164939.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/jac/37.6.1063 doi (NATIONALLICENCE)oxford-10.1093/jac/37.6.1063 Rapid flow cytometric assessment of mecillinam and ampicillin bacterial susceptibility [Elektronische Daten] [Mette Walberg, Peter Gaustad, Harald B. Steen] The effects of antibiotics on Escherichia coli during exponential growth in liquid medium were monitored by flow cytometry measuring cellular DNA content and cell size of individual cells in large numbers as well as cell counts. A statistically significant increase in cellular DNA as well as size was recorded after 20 and 40 min of incubation with the MIC of ampicillin and mecillinam, respectively. The optical density (OD600nm) of treated cultures continued to increase for at least 80 minutes, showing that the increase in cellular DNA and size reflected continued growth and elongation of cells coupled with inhibition of cell division, the latter being confirmed by the cell counting. Since fixation, staining and flow-cytometric analysis can be carried out within a few minutes, the present results suggest that flow cytometry may have potential as a rapid and quantitative technique that can be automated for clinical and experimental susceptibility testing of antibacterial drugs. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Walberg Mette Department of Biophysics, The Norwegian Radium Hospital 0310 Oslo, Norway aut Gaustad Peter Kapt. W. Wilhelmsen og Frues Bakteriologiske Institutt, Rikshospitalet 0027 Oslo, Norway aut Steen Harald B. Department of Biophysics, The Norwegian Radium Hospital 0310 Oslo, Norway aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/6(1996-06), 1063-1075 0305-7453 37:6<1063 1996 37 jac https://doi.org/10.1093/jac/37.6.1063 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.6.1063 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Walberg Mette Department of Biophysics, The Norwegian Radium Hospital 0310 Oslo, Norway aut NATIONALLICENCE 700 1- Gaustad Peter Kapt. W. Wilhelmsen og Frues Bakteriologiske Institutt, Rikshospitalet 0027 Oslo, Norway aut NATIONALLICENCE 700 1- Steen Harald B. Department of Biophysics, The Norwegian Radium Hospital 0310 Oslo, Norway aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/6(1996-06), 1063-1075 0305-7453 37:6<1063 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford