caa a22 4500 397591411 CHVBK 20180308164939.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/jac/38.6.1079 doi (NATIONALLICENCE)oxford-10.1093/jac/38.6.1079 Characterisation of DNA gyrase and measurement of drug accumulation in clinical isolates of Acinetobacter baumannii resistant to fluoroquinolones [Elektronische Daten] [N. J. Moreau, S. Houot, M. L. Joly-Guillou, E. Bergogne-Bérézin] Twelve clinical isolates of Acinetobacter baumannii highly resistant to pefloxacin (MIC ≥ 32 mg/L) and to ciprofloxacin (MIC ≥ 16 mg/L), were studied. A susceptible isolate used as a reference (MIC of 0.032 and 0.25 mg/L for ciprofloxacin and pefloxacin, respectively) accumulated 85 mg of pefloxacin per litre of cell volume within 10 min, from a solution containing 10 mg/L of antibiotic. One resistant isolate accumulated the same amount of pefloxacin, while the 11 others accumulated between 40 and 70 mg/L of cell volume. The differences between reference and resistant isolates with respect to ciprofloxacin and sparfloxacin accumulation were less pronounced. There were no apparent differences in the outer membrane protein profiles of susceptible and resistant isolates. DNA gyrase was isolated from four A. baumannii and the minimum concentration of fluoroquinolones, required to inhibit gyrase-catalysed supercoiling of plasmid DNA was 5- to 80-fold higher for the resistant isolates than for the reference strain. Although most isolates showed some degree of reduced fluoroquinolone accumulation, a DNA gyrase mutation was more likely to be the main mechanism of the high level resistance encountered. © 1996 by the British Society for Antimicrobial Chemotherapy Brief reports nationallicence Moreau N. J. Université Paris VI, L.R.M.A. 15 Rue de l'Ecole de Médecine, 75270 Paris Cedex 06 aut Houot S. Laboratoire de Microbiologie, Hôpital Bichat-Claude Bernard 46 Rue Henri-Huchard, 75877 Paris Cedex 18, France aut Joly-Guillou M. L. Université Paris VI, L.R.M.A. 15 Rue de l'Ecole de Médecine, 75270 Paris Cedex 06 aut Bergogne-Bérézin E. Laboratoire de Microbiologie, Hôpital Bichat-Claude Bernard 46 Rue Henri-Huchard, 75877 Paris Cedex 18, France aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/6(1996-12), 1079-1083 0305-7453 38:6<1079 1996 38 jac https://doi.org/10.1093/jac/38.6.1079 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.6.1079 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moreau N. J. Université Paris VI, L.R.M.A. 15 Rue de l'Ecole de Médecine, 75270 Paris Cedex 06 aut NATIONALLICENCE 700 1- Houot S. Laboratoire de Microbiologie, Hôpital Bichat-Claude Bernard 46 Rue Henri-Huchard, 75877 Paris Cedex 18, France aut NATIONALLICENCE 700 1- Joly-Guillou M. L. Université Paris VI, L.R.M.A. 15 Rue de l'Ecole de Médecine, 75270 Paris Cedex 06 aut NATIONALLICENCE 700 1- Bergogne-Bérézin E. Laboratoire de Microbiologie, Hôpital Bichat-Claude Bernard 46 Rue Henri-Huchard, 75877 Paris Cedex 18, France aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/6(1996-12), 1079-1083 0305-7453 38:6<1079 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford