caa a22 4500 397591667 CHVBK 20180308164940.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/jac/38.1.81 doi (NATIONALLICENCE)oxford-10.1093/jac/38.1.81 Comparison of various macrolides on stimulation of human neutrophil degranulation in vitro [Elektronische Daten] [H. Abdelghaffar, D. Vazifeh, M. T. Labro] Macrolide antibiotics are taken up and concentrated by host cells, particularly phagocytes, and are likely candidates to modify cell functions. In this study, we extended our previous work concerning the effect of three 14-membered-ring macrolides (dirithromycin, erythromycin and erythromycylamine) on human neutrophil exocytosis, and found that three other erythromycin A derivatives (roxithromycin, clarithromycin and the azalide, azithromycin) also triggered neutrophil degranulation in a time- and concentration-dependent manner. After 30 min of incubation, the correlation coefficients for concentration-dependence for roxithromycin were 0.885, 0.739 and 0.750 (P < 0.005) and for clarithromycin were 0.795, 0.599, 0.733 (P < 0.005), respectively, for lysozyme, β-glucuronidase and lactoferrin release. Although the underlying mechanism was not elucidated, these and previous data suggest that intracellular accumulation is a prerequisite. Furthermore, comparison of the characteristics of macrolide-induced exocytosis with those of exocytosis triggered by the synthetic chemotactic stimulus FMLP suggested that different mechanisms are involved. In keeping with this possibility, we showed that combined treatment (macrolides plus FMLP) resulted in totally additive exocytosis of azurophilic but not specific granules. The clinical relevance of our data remains to be ascertained. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Abdelghaffar H. INSERM U294, Service d' Hématologie et d'Immunologie Biologiques, CHU Xavier Bichat, 46 rue Henri Huchard, 75018 Paris, France aut Vazifeh D. INSERM U294, Service d' Hématologie et d'Immunologie Biologiques, CHU Xavier Bichat, 46 rue Henri Huchard, 75018 Paris, France aut Labro M. T. INSERM U294, Service d' Hématologie et d'Immunologie Biologiques, CHU Xavier Bichat, 46 rue Henri Huchard, 75018 Paris, France aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/1(1996-07), 81-93 0305-7453 38:1<81 1996 38 jac https://doi.org/10.1093/jac/38.1.81 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.1.81 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abdelghaffar H. INSERM U294, Service d' Hématologie et d'Immunologie Biologiques, CHU Xavier Bichat, 46 rue Henri Huchard, 75018 Paris, France aut NATIONALLICENCE 700 1- Vazifeh D. INSERM U294, Service d' Hématologie et d'Immunologie Biologiques, CHU Xavier Bichat, 46 rue Henri Huchard, 75018 Paris, France aut NATIONALLICENCE 700 1- Labro M. T. INSERM U294, Service d' Hématologie et d'Immunologie Biologiques, CHU Xavier Bichat, 46 rue Henri Huchard, 75018 Paris, France aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/1(1996-07), 81-93 0305-7453 38:1<81 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford