caa a22 4500 397591683 CHVBK 20180308164940.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/jac/37.4.687 doi (NATIONALLICENCE)oxford-10.1093/jac/37.4.687 Use of the coagulase gene typing method for detection of carriers of methicillin-resistant Staphylococcus aureus [Elektronische Daten] [Christine Lawrence, Marie Cosseron, Olivier Mimoz, Christian Brun-Buisson, Yannick Costa, Kamran Samii, Jean Duval, Ronald Leclercq] Strains of Staphylococcus aureus can be typed on the basis of the polymorphism of the coagulase gene. DNA fragments generated after amplification by polymerase chain reaction (PCR) of the variable region of this gene and digested with the restriction enzyme HaeIII can be compared by their restriction fragment length polymorphism (RFLP). Seventy-nine of 86 (91.8%) methicillin-resistant S. aureus (MRSA) strains isolated in various hospitals had a characteristic RFLP pattern. This pattern differed from those of 32 methicillin-susceptible S. aureus (MSSA). Only one MSSA shared with MRSA the same RFLP pattern. After modification, we applied this method to the rapid detection of MRSA from 255 nasal swabs from patients hospitalized in intensive care units. When screened by plating on Chapman agar, 55 these samples contained MRSA, whereas 40/55 yielded the expected restriction profile after amplification by PCR. No DNA was amplified by PCR in 9/55 samples and restriction profiles were uninterpretable in six cases. When compared to culture, the positive and negative predictive values of the PCR test were 100% and 93%, respectively. The specificity was 100% and the sensitivity was 72.7%. Since control spread of MRSA strains in hospitals is based in part on rapid isolation of carriers, this method which allows detection of epidemic MRSA in nasal swabs within a day could be helpful, though culture would still be necessary to confirm the result. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Lawrence Christine Service de Bactériologie-Virologie -Hygiene; Hôpital Henri Mondor, Université Paris XII 94010 Créteil Cedex; France aut Cosseron Marie Service de Bactériologie-Virologie-Hygiènet; Université Paris Sud 94275 Le Kremlin-Bicêtre Cedex, France aut Mimoz Olivier Service d' Anesthésie-Réanimation, Hôpital du Kremlin -Bicêtre, Université Paris Sud 94275 Le Kremlin-Bicêtre Cedex, France aut Brun-Buisson Christian Service de Réanimation Médicale, Hôpital Henri Mondor, Université Paris XII 94010 Créteil Cedex; France aut Costa Yannick Service de Bactériologie-Virologie-Hygiènet; Université Paris Sud 94275 Le Kremlin-Bicêtre Cedex, France aut Samii Kamran Service d' Anesthésie-Réanimation, Hôpital du Kremlin -Bicêtre, Université Paris Sud 94275 Le Kremlin-Bicêtre Cedex, France aut Duval Jean Service de Bactériologie-Virologie -Hygiene; Hôpital Henri Mondor, Université Paris XII 94010 Créteil Cedex; France aut Leclercq Ronald Service de Bactériologie-Virologie -Hygiene; Hôpital Henri Mondor, Université Paris XII 94010 Créteil Cedex; France aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/4(1996-04), 687-696 0305-7453 37:4<687 1996 37 jac https://doi.org/10.1093/jac/37.4.687 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.4.687 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lawrence Christine Service de Bactériologie-Virologie -Hygiene; Hôpital Henri Mondor, Université Paris XII 94010 Créteil Cedex; France aut NATIONALLICENCE 700 1- Cosseron Marie Service de Bactériologie-Virologie-Hygiènet; Université Paris Sud 94275 Le Kremlin-Bicêtre Cedex, France aut NATIONALLICENCE 700 1- Mimoz Olivier Service d' Anesthésie-Réanimation, Hôpital du Kremlin -Bicêtre, Université Paris Sud 94275 Le Kremlin-Bicêtre Cedex, France aut NATIONALLICENCE 700 1- Brun-Buisson Christian Service de Réanimation Médicale, Hôpital Henri Mondor, Université Paris XII 94010 Créteil Cedex; France aut NATIONALLICENCE 700 1- Costa Yannick Service de Bactériologie-Virologie-Hygiènet; Université Paris Sud 94275 Le Kremlin-Bicêtre Cedex, France aut NATIONALLICENCE 700 1- Samii Kamran Service d' Anesthésie-Réanimation, Hôpital du Kremlin -Bicêtre, Université Paris Sud 94275 Le Kremlin-Bicêtre Cedex, France aut NATIONALLICENCE 700 1- Duval Jean Service de Bactériologie-Virologie -Hygiene; Hôpital Henri Mondor, Université Paris XII 94010 Créteil Cedex; France aut NATIONALLICENCE 700 1- Leclercq Ronald Service de Bactériologie-Virologie -Hygiene; Hôpital Henri Mondor, Université Paris XII 94010 Créteil Cedex; France aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/4(1996-04), 687-696 0305-7453 37:4<687 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford