caa a22 4500 397591705 CHVBK 20180308164940.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/jac/37.1.105 doi (NATIONALLICENCE)oxford-10.1093/jac/37.1.105 Intracellular distribution of ampicillin in murine macrophages infected with Salmonella typhimurium and treated with (3H)ampicillin-loaded nanoparticles [Elektronische Daten] [Olivier Balland, Huguette Pinto-Alphandary, Annie Viron, Edmond Puvion, Antoine Andremont, Patrick Couvreur] The intracellular distribution of (3H polyisohexylcyanoacrylate nanoparticles was studied in murine macrophages (peritoneal cells and the J774 cell line) infected by Salmonella tvphimurium C5, using ultrastructural autoradiography. Ampicillin penetration and retention into the cells obviously increased by means of nanoparticles. After short-term (2-4 h) treatment with the nanoparticle formulation, numerous intracellular bacteria were seen to be in the process of destruction. The tritium labelling was located in the cell cytoplasm and inside vacuoles in which bacteria undergoing degradation were often present. After long-term (12 h) treatment, numerous spherical bodies (d: 100 nm to 500 nm) and larger forms (2 μm) were seen in the vacuoles. Radioactivity was mainly found to be localized on the spherical bodies, indicating marked damaging action of the ampicillin on the bacterial walls. The targeting of ampicillin therefore allowed its penetration into the macrophages and vacuoles infected with S. typhimurium. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Balland Olivier Laboratoire de pharmacie Galénique CNRS, URA 1218, Faculté de Pharmacie, 92296 Châtenay-Malabry Cedex, France aut Pinto-Alphandary Huguette Laboratoire de pharmacie Galénique CNRS, URA 1218, Faculté de Pharmacie, 92296 Châtenay-Malabry Cedex, France aut Viron Annie Unité de Biologie et Ultrastructure du Noyan UPR 272, Institut de Recherches Scientifiques sur le Canfer, BP n° 8, 94801 Villejuif Cedex; France aut Puvion Edmond Unité de Biologie et Ultrastructure du Noyan UPR 272, Institut de Recherches Scientifiques sur le Canfer, BP n° 8, 94801 Villejuif Cedex; France aut Andremont Antoine Laboratoire d' Ecologie Microbienne, Institut Gustave Roussy 94805 Villejuif Cedex; France aut Couvreur Patrick Laboratoire de pharmacie Galénique CNRS, URA 1218, Faculté de Pharmacie, 92296 Châtenay-Malabry Cedex, France aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/1(1996-01), 105-115 0305-7453 37:1<105 1996 37 jac https://doi.org/10.1093/jac/37.1.105 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.1.105 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Balland Olivier Laboratoire de pharmacie Galénique CNRS, URA 1218, Faculté de Pharmacie, 92296 Châtenay-Malabry Cedex, France aut NATIONALLICENCE 700 1- Pinto-Alphandary Huguette Laboratoire de pharmacie Galénique CNRS, URA 1218, Faculté de Pharmacie, 92296 Châtenay-Malabry Cedex, France aut NATIONALLICENCE 700 1- Viron Annie Unité de Biologie et Ultrastructure du Noyan UPR 272, Institut de Recherches Scientifiques sur le Canfer, BP n° 8, 94801 Villejuif Cedex; France aut NATIONALLICENCE 700 1- Puvion Edmond Unité de Biologie et Ultrastructure du Noyan UPR 272, Institut de Recherches Scientifiques sur le Canfer, BP n° 8, 94801 Villejuif Cedex; France aut NATIONALLICENCE 700 1- Andremont Antoine Laboratoire d' Ecologie Microbienne, Institut Gustave Roussy 94805 Villejuif Cedex; France aut NATIONALLICENCE 700 1- Couvreur Patrick Laboratoire de pharmacie Galénique CNRS, URA 1218, Faculté de Pharmacie, 92296 Châtenay-Malabry Cedex, France aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/1(1996-01), 105-115 0305-7453 37:1<105 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford