caa a22 4500 397591845 CHVBK 20180308164941.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/jac/38.1.95 doi (NATIONALLICENCE)oxford-10.1093/jac/38.1.95 Upregulation of reactive oxygen and nitrogen intermediates in Plasmodium berghei infected mice after rescue therapy with chloroquine or artemether [Elektronische Daten] [Javier Prada, Simone Müller, Ulrich Bienzle, Peter G. Kremsner] Plasmodium berghei ANKA infected C57B1/6 mice develop cerebral malaria at a parasitaemia of 15-25%. When parasitaemia reached 10%, P. berghei infected mice were treated with artemether, chloroquine or clindamycin in order to prevent the occurrence of cerebral malaria. Artemether and chloroquine were highly efficient. Functional tests revealed that zymosan stimulated spleen cells from untreated mice with cerebral malaria showed a slight decrease in their capacity to produce reactive oxygen intermediates (ROI) when compared with naive mice. After artemether or chloroquine treatment, the ROI production was significantly enhanced. The interferon-gamma induced production of reactive nitrogen intermediates (RNI) was slightly elevated in mice with cerebral malaria, but markedly elevated in artemether or chloroquine treated mice when compared with naive mice. Moreover, high levels of inducible nitric oxide synthase gene expression could be detected by in-situ hybridization in spleen sections of mice which had been treated with artemether or chloroquine. These findings suggest that increased production of ROI and RNI after chemotherapy may play a protective role for the host during malaria. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Prada Javier Sektion Humanparasitologie, Institut für Tropenmedizin, Universität Tubingen Wilhelmstrasse 27, D-72074, Tübingen aut Müller Simone Institut für Tropenmedizin Engeldamm 62, 10179 Berlin, Germany aut Bienzle Ulrich Institut für Tropenmedizin Engeldamm 62, 10179 Berlin, Germany aut Kremsner Peter G. Sektion Humanparasitologie, Institut für Tropenmedizin, Universität Tubingen Wilhelmstrasse 27, D-72074, Tübingen aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/1(1996-07), 95-102 0305-7453 38:1<95 1996 38 jac https://doi.org/10.1093/jac/38.1.95 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.1.95 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Prada Javier Sektion Humanparasitologie, Institut für Tropenmedizin, Universität Tubingen Wilhelmstrasse 27, D-72074, Tübingen aut NATIONALLICENCE 700 1- Müller Simone Institut für Tropenmedizin Engeldamm 62, 10179 Berlin, Germany aut NATIONALLICENCE 700 1- Bienzle Ulrich Institut für Tropenmedizin Engeldamm 62, 10179 Berlin, Germany aut NATIONALLICENCE 700 1- Kremsner Peter G. Sektion Humanparasitologie, Institut für Tropenmedizin, Universität Tubingen Wilhelmstrasse 27, D-72074, Tübingen aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/1(1996-07), 95-102 0305-7453 38:1<95 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford