caa a22 4500 397591969 CHVBK 20180308164941.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/jac/38.1.21 doi (NATIONALLICENCE)oxford-10.1093/jac/38.1.21 Critical appraisal of E test for the detection of fluoroquinolone resistance [Elektronische Daten] [Roland N. Jones, Meridith E. Erwin, Janet L. Croco] The ability of E test to accurately detect fluoroquinolone resistance was compared with an agar dilution reference method. Nearly 300 isolates belonging to 26 different species (62.5% with documented ciprofloxacin resistance) were tested with ciprofloxacin, fleroxacin, levofloxacin, norfloxacin, ofloxacin, and sparfloxacin. In contrast to earlier reports, E test MIC values for pneumococci and all rapid growing aerobes were routinely higher (approximately 0.5 log2 dilution step) than agar dilution results. The E test stable-gradient method also efficiently indentified fluoroquinolone-resistant subpopulations which were not detected by the reference procedure with the standard inoculum. Categorical agreement for 1710 test comparisons was approximately 90% with no very major, false-susceptible errors. We found the E test to be a valid, reproducible method for fluoroquinolone susceptibility testing that provided quantitative results and produced a conservative (1.6% false-resistant results) assessment of susceptibility of bacteria including isolates of Streptococcus pneumoniae and Pseudomonas aeruginosa to compounds in this antimicrobial class. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Jones Roland N. Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA aut Erwin Meridith E. Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA aut Croco Janet L. Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/1(1996-07), 21-25 0305-7453 38:1<21 1996 38 jac https://doi.org/10.1093/jac/38.1.21 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.1.21 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jones Roland N. Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA aut NATIONALLICENCE 700 1- Erwin Meridith E. Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA aut NATIONALLICENCE 700 1- Croco Janet L. Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/1(1996-07), 21-25 0305-7453 38:1<21 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford