caa a22 4500 397592116 CHVBK 20180308164941.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/jac/38.3.457 doi (NATIONALLICENCE)oxford-10.1093/jac/38.3.457 Formulation of ‘idealized' topical antimicrobial mixtures for use with cultured skin grafts [Elektronische Daten] [Ian Alan Holder, Steven T. Boyce] In order to develop antimicrobial mixtures which provide broad-spectrum antimicrobial activity for use with cultured cell autografts, several individual antimicrobial agents, in concentrations non-toxic for cells in culture, were tested against a variety of bacteria and Candida spp. isolated from burn patients. An agar well diffusion topical assay was used. Antimicrobials active against Gram-positive and Gram-negative bacteria and antifungal agents, individually, were uniformly effective against their respective spectra of organisms. Broad-spectrum antibacterials were uniformly effective against Gram-negative bacteria but their activity varied against Gram-positive bacteria. Adding an agent active against Gram-positive bacteria to all broad spectrum antibacterial agents conferred uniform Gram-positive activity to the mixture. One mixture consisting of specific Gram-negative, Gram-positive and broad spectrum antibacterial agents, was uniformly active against all bacteria tested and the addition of antifungal agents extended the activity to cover Candida spp. without interfering with the mixture's overall antibacterial activity. Another mixture showed either additive or antagonistic activities against the battery of microorganisms tested. Thus, these methods can be used to identify mixtures of antimicrobials, in concentrations non-toxic for cells in culture, that have very broad spectra of antimicrobial activity. Such mixtures should be evaluated in patients when cultured skin grafts are used. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Holder Ian Alan Shriners Burns Institute, and Departments of Surgery and Microbiology University of Cincinnati, Cincinnati, Ohio, USA aut Boyce Steven T. Shriners Burns Institute, and Departments of Surgery and Microbiology University of Cincinnati, Cincinnati, Ohio, USA aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/3(1996-09), 457-463 0305-7453 38:3<457 1996 38 jac https://doi.org/10.1093/jac/38.3.457 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.3.457 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Holder Ian Alan Shriners Burns Institute, and Departments of Surgery and Microbiology University of Cincinnati, Cincinnati, Ohio, USA aut NATIONALLICENCE 700 1- Boyce Steven T. Shriners Burns Institute, and Departments of Surgery and Microbiology University of Cincinnati, Cincinnati, Ohio, USA aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/3(1996-09), 457-463 0305-7453 38:3<457 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford