caa a22 4500 397592183 CHVBK 20180308164941.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/jac/38.6.987 doi (NATIONALLICENCE)oxford-10.1093/jac/38.6.987 The efficacy of antibiotics enhanced by electrical currents against Pseudomonas aeruginosa biofilms [Elektronische Daten] [Jana Jass, Hilary M. Lappin-Scott] Low electrical currents are reported to enhance the activity of tobramycin and biocides against biofilm bacteria. We report that the activity of those antibiotics to which the bacterium is susceptible in its planktonic state may be enhanced by a low electrical current against the more resistant biofilm cells. Pseudomonas aeruginosa biofilms were formed on a dialysis membrane suspended between two parallel electrode plates in an electrical colonisation cell. Ciprofloxacin, polymyxin B and piperacillin were tested on biofilms at ten times their MIC for 12 h in the presence of 0 (control) and 9 mA/cm2 current density. At these concentrations the antibiotics alone reduced the biofilm population, but in the presence of an electrical current the population was further reduced by ciprofloxacin and polymyxin B though not by piperacillin. Exposure of the planktonic bacteria to the same concentrations of antibiotics demonstrated that ciprofloxacin and polymyxin B reduced the population to below 1% after 12 h and below 0.1% after 24 h, while piperacillin was bacteriostatic. The results suggest that the electrical current can enhance the activity against biofilms of only those antibiotics that are effective against planktonic cells. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Jass Jana Department of Biological Sciences, Hatherly Laboratories, University of Exeter, Exeter EX4 4PS, UK aut Lappin-Scott Hilary M. Department of Biological Sciences, Hatherly Laboratories, University of Exeter, Exeter EX4 4PS, UK aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/6(1996-12), 987-1000 0305-7453 38:6<987 1996 38 jac https://doi.org/10.1093/jac/38.6.987 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.6.987 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jass Jana Department of Biological Sciences, Hatherly Laboratories, University of Exeter, Exeter EX4 4PS, UK aut NATIONALLICENCE 700 1- Lappin-Scott Hilary M. Department of Biological Sciences, Hatherly Laboratories, University of Exeter, Exeter EX4 4PS, UK aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/6(1996-12), 987-1000 0305-7453 38:6<987 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford