caa a22 4500 397592329 CHVBK 20180308164942.0 cr unu---uuuuu 161202e199605 xx s 000 0 eng 10.1093/jac/37.5.881 doi (NATIONALLICENCE)oxford-10.1093/jac/37.5.881 pH modulation of aminoglycoside resistance in Staphylococcus epidermidis harbouring 6′-N-aminoglycoside acetyltransferase [Elektronische Daten] [E. Culebras, J. L. Martinez, F. Baquero, J. C. Pérez-Díaz] The kinetic constants of the aminoglycoside-modifying enzyme 6′-N-aminoglycoside acetyltransferase (AAC(6′)IV) from the clinical strain Staphylococcus epidermidis RYC 13036 differed depending on whether tobramycin and amikacin (glucosamine group) or gentamicin and netilmicin (garosamine group) were used as substrates. Acetylation of the glucosamine antibiotics was highly susceptible to substrate inhibition which increased with pH whereas the garosamine group compounds showed limited substrate inhibition over a wide pH range. These differences in activity correlated with MIC values of S. epidermidis RYC 13036 for different aminoglycosides. Aminosugars moiety and pH markedly influenced the AAC(6′)IV-aminoglycoside interactions. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Culebras E. Centro Nactonal de Biotecnologia (CSIC) Campus UAM, Cantoblanco, 28049-Madrid, Spain aut Martinez J. L. Centro Nactonal de Biotecnologia (CSIC) Campus UAM, Cantoblanco, 28049-Madrid, Spain aut Baquero F. Servicio de Microbiologia, Hospital Ramón y Cajal Carretera de Colmenar Km 9,100, 28034-Madrid, Spain aut Pérez-Díaz J. C. Servicio de Microbiologia, Hospital Ramón y Cajal Carretera de Colmenar Km 9,100, 28034-Madrid, Spain aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/5(1996-05), 881-889 0305-7453 37:5<881 1996 37 jac https://doi.org/10.1093/jac/37.5.881 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.5.881 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Culebras E. Centro Nactonal de Biotecnologia (CSIC) Campus UAM, Cantoblanco, 28049-Madrid, Spain aut NATIONALLICENCE 700 1- Martinez J. L. Centro Nactonal de Biotecnologia (CSIC) Campus UAM, Cantoblanco, 28049-Madrid, Spain aut NATIONALLICENCE 700 1- Baquero F. Servicio de Microbiologia, Hospital Ramón y Cajal Carretera de Colmenar Km 9,100, 28034-Madrid, Spain aut NATIONALLICENCE 700 1- Pérez-Díaz J. C. Servicio de Microbiologia, Hospital Ramón y Cajal Carretera de Colmenar Km 9,100, 28034-Madrid, Spain aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/5(1996-05), 881-889 0305-7453 37:5<881 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford