caa a22 4500 397592841 CHVBK 20180308164943.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/jac/38.2.237 doi (NATIONALLICENCE)oxford-10.1093/jac/38.2.237 Pefloxacin penetration into human necrotic pancreatic tissue [Elektronische Daten] [E. Bertazzoni Minelli, A. Benini, A. Muner, C. Bassi, H. Abbas, P. Pederzoli] Antibiotic prophylaxis may be useful in acute necrotising pancreatitis, a disease associated with a considerable incidence of infectious complications. The aim of this study was to assess pefloxacin penetration into necrotic pancreatic tissue during human necrotic pancreatitis. Ten patients (mean age 53·2 ± 17·4 years) with severe acute pancreatitis (mean Ranson score 4·3) were studied. Pefloxacin was administered at a dose of 400 mg bd every 12 h by iv infusion (bolus, 15 min). Intraoperative samples of necrotic pancreatic tissue and blood were collected simultaneously 1, 2, 4·5, 6, 8·5 or 10 h after the last pefloxacin administration in patients treated for 1, 3, 4, 7, 8, 17 or 20 days. Drug concentrations were determined by the microbiological agar-well diffusion method (Escherichio coli Kp 05124 as test micro-organism in Isosensitest Agar). Levels in serum ranged from 2·0 to 9·0 mg/L (at 2 and 6 h, respectively), in necrotic pancreatic tissue from 2·0 to 29·0 μg/g depending on different sampling time. Maximum tissue peak concentrations appeared between 4 and 6 h. The necrotic pancreatic tissue/serum concentration ratio ranged from 0/9 to 5/1, values depending on tissue sample collection. Therapeutic conantrations (20.6μg/g) above the MIC of potentially pathogenic enteric microorganisms were still present in necrotic pancreatic tissue 10 h after the last drug administration. Pefloxacin appeared to concentrate in necrotic pancreatic tissue, without appreciable accumulation after multiple-dose administration. The pefloxacin concentrations in necrotic pancreatic tissue showed high variability, depending on the degree of necrosis. inflammation and sample vascularization. Our results provided evidence of good, prompt penetration of pefloxacin into necrotic pancreatic tissue. Pefloxacin seems to exhibit favourable pharmacokinetic and pharmaccdynamic properties for pancreatic infections. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Minelli E. Bertazzoni Institute of Pharmacology Borgo Roma, 37134 Verona, Italy aut Benini A. Institute of Pharmacology Borgo Roma, 37134 Verona, Italy aut Muner A. Institute of Pharmacology Borgo Roma, 37134 Verona, Italy aut Bassi C. Surgical Department, University Hospital, University of Verona Borgo Roma, 37134 Verona, Italy aut Abbas H. Surgical Department, University Hospital, University of Verona Borgo Roma, 37134 Verona, Italy aut Pederzoli P. Surgical Department, University Hospital, University of Verona Borgo Roma, 37134 Verona, Italy aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/2(1996-08), 237-243 0305-7453 38:2<237 1996 38 jac https://doi.org/10.1093/jac/38.2.237 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.2.237 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Minelli E. Bertazzoni Institute of Pharmacology Borgo Roma, 37134 Verona, Italy aut NATIONALLICENCE 700 1- Benini A. Institute of Pharmacology Borgo Roma, 37134 Verona, Italy aut NATIONALLICENCE 700 1- Muner A. Institute of Pharmacology Borgo Roma, 37134 Verona, Italy aut NATIONALLICENCE 700 1- Bassi C. Surgical Department, University Hospital, University of Verona Borgo Roma, 37134 Verona, Italy aut NATIONALLICENCE 700 1- Abbas H. Surgical Department, University Hospital, University of Verona Borgo Roma, 37134 Verona, Italy aut NATIONALLICENCE 700 1- Pederzoli P. Surgical Department, University Hospital, University of Verona Borgo Roma, 37134 Verona, Italy aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/2(1996-08), 237-243 0305-7453 38:2<237 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford