caa a22 4500 397592914 CHVBK 20180308164944.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/jac/38.6.941 doi (NATIONALLICENCE)oxford-10.1093/jac/38.6.941 Mechanisms responsible for reduced susceptibility to imipenem in Bacteroides fragilis [Elektronische Daten] [R. Edwards, D. Greenwood] The mechanisms responsible for reduced susceptibility to imipenem (MIC 2-16 mg/L) were investigated in eight strains of Bacteroides fragilis. All the strains produced elevated levels of β-lactamase. Four B. fragilis strains produced metallo-β-lactamase capable of marked imipenem hydrolysis, and these enzymes were shown to be responsible for resistance. There was evidence, from testing susceptibility to imipenem in the presence of clavulanic acid, that increased resistance in two strains was associated with susceptibility to β-lactamases other than metallo-enzymes. With the remaining two strains there was no evidence of enzymic breakdown of imipenem. Neither of these strains showed evidence of decreased permeability to nitrocefin as judged by a method which takes into account the substrate concentration in the periplasmic space A low molecular weight PBP, that was not seen in fully sensitive strains of B. fragilis, was detected in four strains in which reduced susceptibility to imipenem was not associated with metallo-β-lacta-mase activity. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Edwards R. Division of Microbiology and PHLS Laboratory, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, UK aut Greenwood D. Division of Microbiology and PHLS Laboratory, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, UK aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/6(1996-12), 941-951 0305-7453 38:6<941 1996 38 jac https://doi.org/10.1093/jac/38.6.941 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.6.941 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Edwards R. Division of Microbiology and PHLS Laboratory, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, UK aut NATIONALLICENCE 700 1- Greenwood D. Division of Microbiology and PHLS Laboratory, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, UK aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/6(1996-12), 941-951 0305-7453 38:6<941 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford