caa a22 4500 397593023 CHVBK 20180308164944.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/jac/37.3.519 doi (NATIONALLICENCE)oxford-10.1093/jac/37.3.519 Comparative internalization and recycling of different amphotericin B formulations by a macrophage-like cell line [Elektronische Daten] [P. Legrand, A. Vertut-Doi, J. Bolard] The amount of amphotericin B (AmB) associated with cultured murine macrophage-like J774 cells, after incubation with various AmB lipid formulations, was determined by absorption spectroscopy. Large, negatively charged, AmB-containing, multilamellar vesicles and small cholesteryl sulphate-AmB complexes both enhanced the amount of AmB associated with J774 cells at 37°C (up to 500-fold the extracellular concentration). In contrast, AmB-containing, small, negatively charged vesicles (AmBisome), positively charged, oligolamellar vesicles and mixed micelles showed a lower association of the antibiotic with cells, compared with AmB added from a solution in dimethylsulphoxide or Fungizone. Experiments performed at 4°C showed a large reduction of AmB uptake for AmB preparations and AmB added from a solution in dimethysulphoxide or Fungizone, suggesting a high percentage of internalization of the antibiotic. Experiments in the presence of cytochalasin B resulted in a decrease of AmB uptake mainly for the preparations of large diameter, suggesting that these formulations were taken up by phagocytosis. A comparative study with Chinese hamster ovary cells, a model of non-phagocytic cells, snowed a reduction in the take up of AmB. This reduction was always more marked when AmB was incorporated in lipid formulations. On the other hand, accumulation of the antibiotic in J774 cells was shown to be followed by its release from the cells in an unbound form, the extent of release depending on the type of vector used. The results suggest that in some cases macrophages can be considered as reservoirs of antibiotic, releasing free AmB in the medium. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Legrand P. L.P.B.C. (URA CNRS 2056), Universite Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris Cedex 05, France aut Vertut-Doi A. L.P.B.C. (URA CNRS 2056), Universite Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris Cedex 05, France aut Bolard J. L.P.B.C. (URA CNRS 2056), Universite Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris Cedex 05, France aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 519-533 0305-7453 37:3<519 1996 37 jac https://doi.org/10.1093/jac/37.3.519 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.3.519 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Legrand P. L.P.B.C. (URA CNRS 2056), Universite Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris Cedex 05, France aut NATIONALLICENCE 700 1- Vertut-Doi A. L.P.B.C. (URA CNRS 2056), Universite Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris Cedex 05, France aut NATIONALLICENCE 700 1- Bolard J. L.P.B.C. (URA CNRS 2056), Universite Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris Cedex 05, France aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 519-533 0305-7453 37:3<519 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford