caa a22 4500 397593082 CHVBK 20180308164944.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/jac/37.4.677 doi (NATIONALLICENCE)oxford-10.1093/jac/37.4.677 A reverse transcriptase-PCR based assay for in-vitro antibiotic susceptibility testing of Chlamydia pneumoniae [Elektronische Daten] [M. A. Khan, C. W. Potter, R. M. Sharrard] Infections caused by Chlamydia spp. are an important cause of human disease, and the accuracy and reproducibility of antimicrobial susceptibility tests for these bacteria could have considerable clinical implications. We have developed a reverse transcriptase PCR (RT-PCR) based method to determine the antibiotic susceptibility of Chlamydia spp., and compared this with conventional tests using immunofluoresence (IF) staining. The MICs of antimicrobial agents for a test strain of Chlamydia pneumoniae were higher by RT-PCR as compared with IF staining, indicating the greater stringency of the former method. Using RT-PCR, doxycycline and tetracycline were the most active agents (MIC 1 mg/L), followed by erythromycin (1.6 mg/L), and ciprofloxacin (16 mg/L). Neither trimethoprim nor sulphamethoxazole (400 mg/L) inhibited growth as assessed by both techniques. The RT-PCR based method may thus represent an improved and less time consuming assay for in-vitro determination of the antibiotic susceptibility of Chlamydia spp. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Khan M. A. Department of Microbiology University of Sheffield Beech Hill Road, Sheffield S10 2RX, UK aut Potter C. W. Institute for Cancer Studies, Medical School, University of Sheffield, Beech Hill Road, Sheffield S10 2RX, UK, Beech Hill Road, Sheffield S10 2RX, UK aut Sharrard R. M. Institute for Cancer Studies, Medical School, University of Sheffield, Beech Hill Road, Sheffield S10 2RX, UK, Beech Hill Road, Sheffield S10 2RX, UK aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/4(1996-04), 677-685 0305-7453 37:4<677 1996 37 jac https://doi.org/10.1093/jac/37.4.677 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.4.677 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khan M. A. Department of Microbiology University of Sheffield Beech Hill Road, Sheffield S10 2RX, UK aut NATIONALLICENCE 700 1- Potter C. W. Institute for Cancer Studies, Medical School, University of Sheffield, Beech Hill Road, Sheffield S10 2RX, UK, Beech Hill Road, Sheffield S10 2RX, UK aut NATIONALLICENCE 700 1- Sharrard R. M. Institute for Cancer Studies, Medical School, University of Sheffield, Beech Hill Road, Sheffield S10 2RX, UK, Beech Hill Road, Sheffield S10 2RX, UK aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/4(1996-04), 677-685 0305-7453 37:4<677 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford