caa a22 4500 397593147 CHVBK 20180308164944.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/jac/37.3.535 doi (NATIONALLICENCE)oxford-10.1093/jac/37.3.535 HIV-induced cytopathology and viral load in a pentamidine-treated lymphocytic cell line [Elektronische Daten] [Myra A. Arnott, John Hay, John F. Peutherer] Preincubation of CD4 lymphocytes with pentamidine isethionate at a concentration of 1.5 mg/L then removal from incubation medium prior to addition of HIV-1, or incubation of cells with the drug and vims simultaneously, increased HIV-1 DNA load but reduced p24 antigen release. The number of syncytia generated was not affected by the presence of pentamidine. The extent of balloon degeneration of cells was greater, however, although this was not associated with a discernable increase in cellular necrosis or reduction in cell viability. This suggests that drug-treatment resulted in an increased load of intracytoplasmic (but not necessarily integrated) forms of HIV-1; this may explain the lower levels of antigen produced and also the balloon degeneration of treated cells, a phenomenon previously observed with other retroviruses. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Arnott Myra A. Department of Medical Microbiology, The University of Edinburgh Medical School Teviot Place, Edinburgh, EH8 9AG, UK aut Hay John Department of Ophthalmology, Tennent Institute, University of Glasgow Glasgow, G11 6NT, UK aut Peutherer John F. Department of Medical Microbiology, The University of Edinburgh Medical School Teviot Place, Edinburgh, EH8 9AG, UK aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 535-543 0305-7453 37:3<535 1996 37 jac https://doi.org/10.1093/jac/37.3.535 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.3.535 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arnott Myra A. Department of Medical Microbiology, The University of Edinburgh Medical School Teviot Place, Edinburgh, EH8 9AG, UK aut NATIONALLICENCE 700 1- Hay John Department of Ophthalmology, Tennent Institute, University of Glasgow Glasgow, G11 6NT, UK aut NATIONALLICENCE 700 1- Peutherer John F. Department of Medical Microbiology, The University of Edinburgh Medical School Teviot Place, Edinburgh, EH8 9AG, UK aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 535-543 0305-7453 37:3<535 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford