caa a22 4500 39759321X CHVBK 20180308164944.0 cr unu---uuuuu 161202e199612 xx s 000 0 eng 10.1093/jac/38.6.977 doi (NATIONALLICENCE)oxford-10.1093/jac/38.6.977 Pharmacodynamics and pharmacokinetics of A-80556 using microdialysis in a Streptococcus pneumoniae meningitis model [Elektronische Daten] [Christopher J. Destache, Catherine B. Pakiz, Anne M. Stoysich] Using microdialysis in a rabbit model of Streptococcus pneumoniae meningitis, the pharmacokinetics and pharmacodynamics of a new fluoroquinolone, A-80556, were determined. A-80556 (10 mg/kg iv) was administered to four rabbits. Saline was given to four separate control animals. A microdialysis probe perfused the CSF (2 μL/min) and effluent was collected at 0-0.25, 0.25-1, 1-2, 2-4, and 4-6 h after injection of A-80556. Seven blood samples were collected and analyzed by HPLC. At 0, 2, 4, and 6h 300 μL of CSF was withdrawn for pharmacodynamic measurements. Plasma A-80556 concentrations demonstrated AUC0-∞.K 2.40 ± 0.272 μg.h/mL; T½β 1.07 ± 0.011 h; Vd 6.35 ± 0.50 L/kg; and Cl 4.23 ± 0.48 L/h.kg. Penetration into the CSF was 18.21%. Pharmacodynamics using time-kill curves showed a 3-log reduction in bacterial counts in CSF at 2 h after administration continuing for the remaining four hours of the experiment. These results demonstrate that microdialysis can be used for determination of drug penetration and efficacy in experimental S;. pneumoniae meningitis. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Destache Christopher J. Creighton University School of Pharmacy and Allied Health Professions and Medicine, 2500 California Plaza, Omaha, NE 68178, USA aut Pakiz Catherine B. Creighton University School of Pharmacy and Allied Health Professions and Medicine, 2500 California Plaza, Omaha, NE 68178, USA aut Stoysich Anne M. Creighton University School of Pharmacy and Allied Health Professions and Medicine, 2500 California Plaza, Omaha, NE 68178, USA aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/6(1996-12), 977-985 0305-7453 38:6<977 1996 38 jac https://doi.org/10.1093/jac/38.6.977 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.6.977 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Destache Christopher J. Creighton University School of Pharmacy and Allied Health Professions and Medicine, 2500 California Plaza, Omaha, NE 68178, USA aut NATIONALLICENCE 700 1- Pakiz Catherine B. Creighton University School of Pharmacy and Allied Health Professions and Medicine, 2500 California Plaza, Omaha, NE 68178, USA aut NATIONALLICENCE 700 1- Stoysich Anne M. Creighton University School of Pharmacy and Allied Health Professions and Medicine, 2500 California Plaza, Omaha, NE 68178, USA aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/6(1996-12), 977-985 0305-7453 38:6<977 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford