caa a22 4500 397593481 CHVBK 20180308164945.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/jac/37.3.501 doi (NATIONALLICENCE)oxford-10.1093/jac/37.3.501 Activity of rifabutin, clarithromycin, ethambutol, sparfloxacin and amikacin, alone and in combination, against Mycobacterium avium complex in human macrophages [Elektronische Daten] [Isabelle Pellegrin, Jeanne Maugein, Catherine Lapeyre, Pascal Barbeau, Bernard Leng, Jean-Luc Pellegrin] Disseminated infection with Mycobacterium avium complex (MAC) in patients with AIDS is currently treated with a combination of antimycobacterial agents in order to prevent the selection of resistant mutant strains. Although clinical and microbiological responses can generally be achieved within a few weeks, relapses are common and require modification of the combination regimen or identification of effective alternate therapies. In this study we investigated the activities of rifabutin 0.5 mg/L, sparfloxacin 1 mg/L, clarithromycin 4 mg/L, amikacin 16mg/L and ethambutol 2 mg/L, alone and in combination, against nine strains of M. avium isolated from the blood of patients with AIDS in order to identify regimens with the greatest therapeutic potential. Macrophages derived from human monocytes were infected with M. avium and inoculated with a single drug or a combination of drugs; cfu counts were performed at 0, 4 and 7 days after infection. At day 4 and at day 7, the combination of rifabutin, clarithromycin, amikacin and sparfloxacin displayed the highest degree of activity. However, the activity did not differ significantly from that of the combination of rifabutin, clarithromycin and ethambutol. The results of this study confirm the activity of combinations including rifabutin and clarithromycin (± ethambutol) in human monocyte-derived macrophages and suggest potentially useful associations in incorporating sparfloxacin and amikacin. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Pellegrin Isabelle Laboratoire de Bactériologie, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut Maugein Jeanne Laboratoire de Bactériologie, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut Lapeyre Catherine Laboratoire de Bactériologie, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut Barbeau Pascal Clinique de Médecine Interne et Maladies Infectieuses, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut Leng Bernard Clinique de Médecine Interne et Maladies Infectieuses, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut Pellegrin Jean-Luc Clinique de Médecine Interne et Maladies Infectieuses, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 501-510 0305-7453 37:3<501 1996 37 jac https://doi.org/10.1093/jac/37.3.501 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.3.501 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pellegrin Isabelle Laboratoire de Bactériologie, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut NATIONALLICENCE 700 1- Maugein Jeanne Laboratoire de Bactériologie, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut NATIONALLICENCE 700 1- Lapeyre Catherine Laboratoire de Bactériologie, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut NATIONALLICENCE 700 1- Barbeau Pascal Clinique de Médecine Interne et Maladies Infectieuses, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut NATIONALLICENCE 700 1- Leng Bernard Clinique de Médecine Interne et Maladies Infectieuses, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut NATIONALLICENCE 700 1- Pellegrin Jean-Luc Clinique de Médecine Interne et Maladies Infectieuses, Hôpital Haut-Léveque (CHR Bordeaux) 33604 Pessac, France aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 501-510 0305-7453 37:3<501 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford