caa a22 4500 397593511 CHVBK 20180308164945.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/jac/37.3.433 doi (NATIONALLICENCE)oxford-10.1093/jac/37.3.433 Imipenem and cephem resistant Pseudomonas aeruginosa carrying plasmids coding for class B β-lactamase [Elektronische Daten] [S. Minami, H. Akama, Y. Watanabe, H. Narita, S. Iyobe, S. Mitsuhashi] From October 1988 to January 1992, nine isolates of Pseudomonas aeruginosa carrying transferable plasmids encoding imipenem-hydrolyzing β-lactamase (pI = c. 9.5) were recovered from nine different patients in a neurosurgical ward of a hospital in Japan. The β-lactamase activities of the sonicated extracts from the transconjugants were inhibited by EDTA and this was partially reversible by the addition of zinc cation. The substrate specificity and pI of the β-lactamase were similar to those of the metallo β-lactamases from P. aeruginosa and Serratia marcescens TN9106. All strains were resistant to imipenem, carbenicillin and antipseudomonal cephems including ceftazidime, cefsulodin, cefpirome, while four and five strains were susceptible to piperacillin and aztreonam, respectively. Both low level imipenem resistance and high level cephem resistance were co-transferred with the production of metallo β-lactamase, while resistance to piperacillin, aztreonam, and high level imipenem-resistance were not selected. Production of chromosomal cephalosporinase in piperacillin resistant strains was derepressed, and production of outer membrane protein of D2 was diminished in highly imipenem resistant strains. Six strains were isolated in 1991, and the amounts of antipseudomonal agents, especially imipenem, used in the neurosurgical ward increased markedly in this year. Only three of the nine isolates had the same serotype, pyocin type and phage type. Our results suggest that the repeated isolation of imipenem and cephem-resistant P. aeruginosa producing metallo β-lactamase was related to the high usage of antipseudomonal β-lactam antibiotics such as imipenem, and was exacerbated by the dissemination of a plasmid. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Minami S. Toyama-city hospital 292 Imaizumi, Toyama-city, Toyama, Japan aut Akama H. Research Laboratories, Toyama Chemical Co., Ltd. 2-4-1 Shimo-okui, Toyama-city, Toyama 930, Japan aut Watanabe Y. Research Laboratories, Toyama Chemical Co., Ltd. 2-4-1 Shimo-okui, Toyama-city, Toyama 930, Japan aut Narita H. Research Laboratories, Toyama Chemical Co., Ltd. 2-4-1 Shimo-okui, Toyama-city, Toyama 930, Japan aut Iyobe S. Laboratory of Drug Resistance in Bacteria, School of Medicine, Gunma University Showa-machi, Maebashi, Gunma, Japan aut Mitsuhashi S. Episome Institute 2220 Kogure, Fujimi-mura, Seta-gun Gunma, Japan aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 433-444 0305-7453 37:3<433 1996 37 jac https://doi.org/10.1093/jac/37.3.433 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.3.433 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Minami S. Toyama-city hospital 292 Imaizumi, Toyama-city, Toyama, Japan aut NATIONALLICENCE 700 1- Akama H. Research Laboratories, Toyama Chemical Co., Ltd. 2-4-1 Shimo-okui, Toyama-city, Toyama 930, Japan aut NATIONALLICENCE 700 1- Watanabe Y. Research Laboratories, Toyama Chemical Co., Ltd. 2-4-1 Shimo-okui, Toyama-city, Toyama 930, Japan aut NATIONALLICENCE 700 1- Narita H. Research Laboratories, Toyama Chemical Co., Ltd. 2-4-1 Shimo-okui, Toyama-city, Toyama 930, Japan aut NATIONALLICENCE 700 1- Iyobe S. Laboratory of Drug Resistance in Bacteria, School of Medicine, Gunma University Showa-machi, Maebashi, Gunma, Japan aut NATIONALLICENCE 700 1- Mitsuhashi S. Episome Institute 2220 Kogure, Fujimi-mura, Seta-gun Gunma, Japan aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 433-444 0305-7453 37:3<433 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford