caa a22 4500 397593546 CHVBK 20180308164945.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/jac/38.3.435 doi (NATIONALLICENCE)oxford-10.1093/jac/38.3.435 Expression of Aeromonas caviae bla genes in Escherichia coli [Elektronische Daten] [Sameera Sayeed, Jon. R. Saunders, Clive Edwards, John E. Corkill, C. Anthony Hart] An isolate of Aeromonas caviae 035 carried a 55.5 kb self-transferable plasmid. Transfer of the plasmid to Escherichia coli K12 resulted in the expression of a TEM-like β-lactamase that was not expressed in parental A. caviae. The bla gene sequence was detectable by DNA hybridization and PCR amplification of the plasmid when extracted from parental A. caviae or from E. coli K. 12 transconjugants. Sequence analysis of the bla genes revealed in each case an 858 bp open reading frame, differing from the TEM-1 sequence by five nucleotide substitutions. Only one of these resulted in amino acid substitution (at position 162 serine→arginine). The sequence most closely resembles that of TEM-12. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Sayeed Sameera Department of Genetics and Microbiology aut Saunders Jon. R. Department of Genetics and Microbiology aut Edwards Clive Department of Genetics and Microbiology aut Corkill John E. Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool PO Box 147, Liverpool, L69 3BX UK aut Hart C. Anthony Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool PO Box 147, Liverpool, L69 3BX UK aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/3(1996-09), 435-441 0305-7453 38:3<435 1996 38 jac https://doi.org/10.1093/jac/38.3.435 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.3.435 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sayeed Sameera Department of Genetics and Microbiology aut NATIONALLICENCE 700 1- Saunders Jon. R. Department of Genetics and Microbiology aut NATIONALLICENCE 700 1- Edwards Clive Department of Genetics and Microbiology aut NATIONALLICENCE 700 1- Corkill John E. Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool PO Box 147, Liverpool, L69 3BX UK aut NATIONALLICENCE 700 1- Hart C. Anthony Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool PO Box 147, Liverpool, L69 3BX UK aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/3(1996-09), 435-441 0305-7453 38:3<435 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford