caa a22 4500 397593651 CHVBK 20180308164946.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/jac/37.6.1103 doi (NATIONALLICENCE)oxford-10.1093/jac/37.6.1103 Influence of inoculum, medium and serum on the in-vitro susceptibility of coagulase-negative staphylococci to teicoplanin and vancomycin [Elektronische Daten] [H. F. Kennedy, D. V. Seal] The susceptibility to teicoplanin of 100 coagulase negative staphylococci, predominantly isolated from intravenous catheter tips and exit sites was determined by agar dilution on IsoSensitest media with and without the addition of 20% inactivated horse serum using an inoculum of 104cfu/spot. Incorporation of serum resulted in a three-fold increase in the geometric mean MIC and a fourfold increase in the geometric mean MBC. Further tests, performed in DST and IsoSensitest medium supplemented with 20% inactivated horse serum, resulted in a wide variation in teicoplanin MICs with differences in the geometric mean MICs being up to 8.8-fold in serum media compared to unsupplemented media, whereas the MICs of vancomycin were unaffected. Under the various experimental conditions used, the susceptibility of the coagulase negative staphylococci to teicoplanin varied from 31-100%, while all were consistently susceptible to vancomycin. We therefore recommend that teicoplanin MICs be determined in the presence of serum as these are better related to serum drug levels and reflect more accurately the conditions in vivo. Trials of teicoplanin as monotherapy are required to provide further insight into the relationship between its in-vitro antibacterial activity and its clinical efficacy as the drug is easier to administer, better tolerated and less toxic than vancomycin which might compensate for its reduced activity in the presence of serum. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Kennedy H. F. Department of Microbiology, Royal Hospital for Sick Children, Yorkhill NHS Trust, Glasgow G3 8SJ, UK aut Seal D. V. Department of Microbiology, Royal Hospital for Sick Children, Yorkhill NHS Trust, Glasgow G3 8SJ, UK aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/6(1996-06), 1103-1109 0305-7453 37:6<1103 1996 37 jac https://doi.org/10.1093/jac/37.6.1103 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.6.1103 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kennedy H. F. Department of Microbiology, Royal Hospital for Sick Children, Yorkhill NHS Trust, Glasgow G3 8SJ, UK aut NATIONALLICENCE 700 1- Seal D. V. Department of Microbiology, Royal Hospital for Sick Children, Yorkhill NHS Trust, Glasgow G3 8SJ, UK aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/6(1996-06), 1103-1109 0305-7453 37:6<1103 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford