caa a22 4500 397593759 CHVBK 20180308164946.0 cr unu---uuuuu 161202e199611 xx s 000 0 eng 10.1093/jac/38.5.787 doi (NATIONALLICENCE)oxford-10.1093/jac/38.5.787 Is the uptake of pefloxacin in human blood monocytes a simple diffusion process? [Elektronische Daten] [E. Memin, G. Panteix, A. Revol] Effective therapy of infections caused by facultative intracellular micro-organisms, which persist after ingestion by mononuclear phagocytic cells, requires antibiotics which inactivate these intraphagocytic bacteria. A previous study demonstrated that intracellular concentrations of normethylpefloxacin, Ci, were the result of simple diffusion and an active efflux involving organic anion transporters. In our work, we studied pefloxacin transport in human monocytes. A kinetic approach was followed to establish the processes involved. Extracellular concentrations, Ce, corresponding to in-vivo pharmacological levels of the drug were used. Uptake of the antibiotic was assessed principally by HPLC. Pefloxacin was accumulated by the cells (Ci/Ce =3). The uptake was rapid, non saturable, reversible and temperature dependent. At 4°C, the Ci/Ce was equal to 1. At high temperatures, an active and initial process appeared that was not visible at lower temperatures and disappeared in presence of energetic metabolism inhibitor: At 42°C, NaCN and 2.4 DNP (1 mm) reduced the initial transport but they had no significant effect on the subsequent curve. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Memin E. Centre Hospitalier Lyon Sud, Service de Biochimie Bat 3B, Secteur J. Courmont, 69310 Pierre Bénite Panteix G. Centre Hospitalier Lyon Sud, Service de Biochimie Bat 3B, Secteur J. Courmont, 69310 Pierre Bénite aut Revol A. Centre Hospitalier Lyon Sud, Service de Biochimie Bat 3B, Secteur J. Courmont, 69310 Pierre Bénite aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/5(1996-11), 787-798 0305-7453 38:5<787 1996 38 jac https://doi.org/10.1093/jac/38.5.787 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.5.787 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Memin E. Centre Hospitalier Lyon Sud, Service de Biochimie Bat 3B, Secteur J. Courmont, 69310 Pierre Bénite NATIONALLICENCE 700 1- Panteix G. Centre Hospitalier Lyon Sud, Service de Biochimie Bat 3B, Secteur J. Courmont, 69310 Pierre Bénite aut NATIONALLICENCE 700 1- Revol A. Centre Hospitalier Lyon Sud, Service de Biochimie Bat 3B, Secteur J. Courmont, 69310 Pierre Bénite aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/5(1996-11), 787-798 0305-7453 38:5<787 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford