caa a22 4500 397593775 CHVBK 20180308164946.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/jac/38.1.39 doi (NATIONALLICENCE)oxford-10.1093/jac/38.1.39 Carbapenem resistance in Pseudomonas aeruginosa from cystic fibrosis patients [Elektronische Daten] [S. Ballestero, A. Fern´ndez-Rodriguez, R. Villaverde, H. Escobar, J. C. Pérez-Diaz, F. Baquero] The evolution of imipenem resistance was evaluated in Pseudomonas aeruginosa sequentially isolated from 42 patients with cystic fibrosis. Susceptibility was determined using a commercial microdilution system and imipenem resistance was confirmed by the agar dilution technique. Resistance to imipenem increased during the years from 1988 to 1992. A total of 12 patients (28.5%) carried resistant strains (11.6% of the total P. aeruginosa isolates) but only two of them were treated with the carbapenem. The other patient under imipenem treatment did not harbour resistant isolates. Sixty-four percent of the imipenem resistant isolates were also meropenem resistant and showed low susceptibility to the other β-lactams and tobramycin and amikacin. Twenty-one strains were selected for biochemical study. Imipenem susceptible strains showed normal OprD in two strains and diminished OprD in two more. Five strains with MIC of imipenem of 4-8 mg‐L lacked OprD while another two had a band with decreased density. All strains with MIC higher than 8 completely lacked this band in western-blot analysis. Imipenem MICs of 0.5-2 mg/L only slightly increased to 1-4 mg/L when a pattern of β-lactamase derepression was observed. While those with imipenem MICs between 8-16 mg/L increased the imipenem MIC to 16-64 mg/L in the population with a β-lactamase derepression phenotype. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence Ballestero S. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut Fern´ndez-Rodriguez A. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut Villaverde R. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut Escobar H. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut Pérez-Diaz J. C. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut Baquero F. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/1(1996-07), 39-45 0305-7453 38:1<39 1996 38 jac https://doi.org/10.1093/jac/38.1.39 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.1.39 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ballestero S. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut NATIONALLICENCE 700 1- Fern´ndez-Rodriguez A. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut NATIONALLICENCE 700 1- Villaverde R. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut NATIONALLICENCE 700 1- Escobar H. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut NATIONALLICENCE 700 1- Pérez-Diaz J. C. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut NATIONALLICENCE 700 1- Baquero F. Servicios de Microbiología y Pediatría, Unidad de Fibrosis Quística, Hospital Ramón y Cajal, Crta Colmenar Viejo km 9,100. 28034 Madrid, Spain aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/1(1996-07), 39-45 0305-7453 38:1<39 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford