caa a22 4500 397593910 CHVBK 20180308164947.0 cr unu---uuuuu 161202e199601 xx s 000 0 eng 10.1093/jac/37.1.65 doi (NATIONALLICENCE)oxford-10.1093/jac/37.1.65 Bleomycin resistance in Staphylococcus aureus clinical isolates [Elektronische Daten] [Dimitra Gennimata, Julian Davies, S. Tsiftsoglour] Among clinical isolates of Staphylococcus aureus tested for resistance to the antibiotic bleomycin, 197 were found to be resistant; most of them were also resistant to tobramycin and contained plasmids. DNA dot-blot hybridization analysis of the bleomycin resistant isolates with an 171 bp probe derived from plasmid pUB110 indicated that 43 strains (22%) carried pUBI10-like bleomycin resistance DNA sequences. Analysis of bacterial cell lysates derived from the bleomycin resistant isolates indicated that many contained bleomycin-binding proteins (BBP), that prevent DNA damage by the antibiotic. Of 13 strains that were analysed by DNA gel electrophoresis and Southern blot DNA hybridization, six were found to carry pUBI10-like bleomycin resistance DNA sequences. These studies indicate that there may be more than one genetic determinant for bleomycin resistance in S. aureus whose DNA is protected. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Gennimata Dimitra Laboratory of Pharmacology, Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki Thessaloniki 546 06 aut Davies Julian Microbial Engineering Unit, Institut Pasteur Paris, France aut Tsiftsoglour S. Laboratory of Pharmacology, Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki Thessaloniki 546 06 aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/1(1996-01), 65-75 0305-7453 37:1<65 1996 37 jac https://doi.org/10.1093/jac/37.1.65 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.1.65 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gennimata Dimitra Laboratory of Pharmacology, Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki Thessaloniki 546 06 aut NATIONALLICENCE 700 1- Davies Julian Microbial Engineering Unit, Institut Pasteur Paris, France aut NATIONALLICENCE 700 1- Tsiftsoglour S. Laboratory of Pharmacology, Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki Thessaloniki 546 06 aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/1(1996-01), 65-75 0305-7453 37:1<65 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford