caa a22 4500 397593953 CHVBK 20180308164947.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/jac/38.3.465 doi (NATIONALLICENCE)oxford-10.1093/jac/38.3.465 Cytotoxicity of macrolide antibiotics in a cultured human liver cell line [Elektronische Daten] [Matti Viluksela, Petri J. Vainio, Raimo K. Tuominen] Cytotoxicity of erythromycin base, erythromycin estolate, erythromycin-11,12-cyclic carbonate, roxithromycin, clarithromycin and azithromycin was compared in cultured human non-malignant Chang liver cells using reduction of 3-{4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and cellular protein concentration as end points of toxicity. Erythromycin estolate was the most toxic macrolide in all tests differing clearly from all the other macrolides studied. Erythromycin-11,12-cyclic carbonate was also more toxic than the other macrolides. Roxithromycin and clarithromycin were the next toxic derivatives, while erythromycin base and azithromycin were least toxic. Thus, cytotoxicity of the new semisynthetic macrolides, roxithromycin, clarithromycin and azithromycin, is not substantially different from that of erythromycin base. In view of the low level of hepatotoxicity of macrolides hitherto reported in humans, the results do not suggest any substantial risk for hepatic disorders related to the use of azithromycin, clarithromycin and roxithromycin. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Viluksela Matti Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Helsinki P.O. Box 8, FIN-00014 University of Helsinki aut Vainio Petri J. Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Helsinki P.O. Box 8, FIN-00014 University of Helsinki aut Tuominen Raimo K. Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Helsinki P.O. Box 8, FIN-00014 University of Helsinki aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/3(1996-09), 465-473 0305-7453 38:3<465 1996 38 jac https://doi.org/10.1093/jac/38.3.465 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.3.465 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Viluksela Matti Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Helsinki P.O. Box 8, FIN-00014 University of Helsinki aut NATIONALLICENCE 700 1- Vainio Petri J. Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Helsinki P.O. Box 8, FIN-00014 University of Helsinki aut NATIONALLICENCE 700 1- Tuominen Raimo K. Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Helsinki P.O. Box 8, FIN-00014 University of Helsinki aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/3(1996-09), 465-473 0305-7453 38:3<465 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford