caa a22 4500 397593961 CHVBK 20180308164947.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/jac/38.2.193 doi (NATIONALLICENCE)oxford-10.1093/jac/38.2.193 A new time-kill method of assessing the relative efficacy of antimicrobial agents alone and in combination developed using a representative β-lactam, aminoglycoside and fluoroquinolone [Elektronische Daten] [A. P. MacGowan, M. Wootton, A. J. Hedges, K. E. Bowker, H. A. Holt, D. S. Reeves] A time-kill curve employing nine sampling times over 6 h was used to provide data which were then used to develop a theoretical (best-fit) curve. From the theoretical curve parameters describing the rate of kill (α), time from addition of antibiotic to initiation of killing (d) and a function of the degree of killing observed (Ym/ Yo) were defined. The area-under-the-curve (AUC) was calculated from the theoretical curve. The variability of each parameter was assessed using a theoretical curve to fit the data from experiments done on three occasions and in triplicate. In terms of the parameters α, d, Ym/Yo and AUC, no synergy was demonstrated with combinations of piperacillin/tazobactam plus ciprofloxacin or gentamicin when compared with single antibiotics. The AUC represents the best summary parameter of a time-kill curve but should be supported by other parameters describing the best-fit curve. © 1996 by the British Society for Antimicrobial Chemotherapy Original articles nationallicence MacGowan A. P. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut Wootton M. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut Hedges A. J. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut Bowker K. E. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut Holt H. A. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut Reeves D. S. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut Journal of Antimicrobial Chemotherapy Oxford University Press 38/2(1996-08), 193-203 0305-7453 38:2<193 1996 38 jac https://doi.org/10.1093/jac/38.2.193 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/38.2.193 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- MacGowan A. P. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut NATIONALLICENCE 700 1- Wootton M. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut NATIONALLICENCE 700 1- Hedges A. J. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut NATIONALLICENCE 700 1- Bowker K. E. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut NATIONALLICENCE 700 1- Holt H. A. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut NATIONALLICENCE 700 1- Reeves D. S. Bristol Centre for Antimicrobial Research & Evaluation, Southmead Health Services NHS Trust and the University of Bristol, Department of Medical Microbiology, Southmead Hospital, Westbury-on-Trym, Bristol BS10 5NB, UK aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 38/2(1996-08), 193-203 0305-7453 38:2<193 1996 38 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford