caa a22 4500 397593996 CHVBK 20180308164947.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/jac/37.3.483 doi (NATIONALLICENCE)oxford-10.1093/jac/37.3.483 In-vitro activity of azithromycin against intracellular Helicobacter pylori [Elektronische Daten] [Kristina Hultén, Otto Cars, Eva Hjelm, Lars Engstrand] We studied the effect in vitro of azithromycin on the clinical strain Helicobacter pylori H:72 growing intracellularly in monolayers of HEp-2 epithelial cells. After using gentamicin to eradicate extracellular bacteria, different concentrations of azithromycin were added to the infected cells and samples were taken after 0, 4, 8 and 24 h. Infected cells not exposed to antibiotic were included as controls. The MIC of azithromycin to the H. pylori was 0.25 mg/L and the MBC 0.5 mg/L in a broth dilution plate count method. A bactericidal effect was observed on intracellular H. pylori, with inhibition increasing with increasing azithromycin concentrations. However, extracellular concentrations of 200 × MBC were necessary to achieve intracellular killing . Our results show that azithromycin is active against intracellular H. pylori suggesting that it might be possible to exploit this activity when treating infections due to the organism. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Hultén Kristina Department of Infectious Diseases and Clinical Microbiology, University Hospital, Uppsala, Sweden aut Cars Otto Department of Infectious Diseases and Clinical Microbiology, University Hospital, Uppsala, Sweden aut Hjelm Eva Department of Infectious Diseases and Clinical Microbiology, University Hospital, Uppsala, Sweden aut Engstrand Lars Department of Infectious Diseases and Clinical Microbiology, University Hospital, Uppsala, Sweden aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 483-489 0305-7453 37:3<483 1996 37 jac https://doi.org/10.1093/jac/37.3.483 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.3.483 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hultén Kristina Department of Infectious Diseases and Clinical Microbiology, University Hospital, Uppsala, Sweden aut NATIONALLICENCE 700 1- Cars Otto Department of Infectious Diseases and Clinical Microbiology, University Hospital, Uppsala, Sweden aut NATIONALLICENCE 700 1- Hjelm Eva Department of Infectious Diseases and Clinical Microbiology, University Hospital, Uppsala, Sweden aut NATIONALLICENCE 700 1- Engstrand Lars Department of Infectious Diseases and Clinical Microbiology, University Hospital, Uppsala, Sweden aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 483-489 0305-7453 37:3<483 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford