caa a22 4500 39759402X CHVBK 20180308164947.0 cr unu---uuuuu 161202e199603 xx s 000 0 eng 10.1093/jac/37.3.423 doi (NATIONALLICENCE)oxford-10.1093/jac/37.3.423 Enzyme kinetics and biochemical analysis of ImiS, the metallo-β-lactamase from Aeromonas sobria 163a [Elektronische Daten] [Timothy R. Walsh, Steven Gamblin, David C. Emery, Alasdair P. MacGowan, Peter M. Bennett] The metallo-β-lactamase from Aeromonas sobria 163a, ImiS, was isolated in a two stage purification procedure using protein affinity columns. Enzyme kinetics show that ImiS hydrolyses the carbapenems but displays poor activity against other β-lactams. ImiS possesses the narrowest spectrum of activity of the Group 3 enzymes that have been analysed. Sequencing of the 40 N-terminal amino acids show this region to be identical to that of the CphA metallo-βlactamase from Aeromonas hydrophila (Massidda, Rossolini & Satta, 1991). Light scattering analysis indicates that ImiS is functionally active as a monomer. © 1996 The British Society for Antimicrobial Chemotherapy Original articles nationallicence Walsh Timothy R. Department of Microbiology and Pathology, School of Medical Sciences, University of Bristol Bristol BS8 JTD, UK aut Gamblin Steven National Medical Research Centre The Ridgeway, Millhill, London NW7 1AA, UK aut Emery David C. National Medical Research Centre The Ridgeway, Millhill, London NW7 1AA, UK aut MacGowan Alasdair P. Regional Antimicrobial Reference Laboratory, Southmead Health Services NHS Trust, Bristol BS10 5NB, UK aut Bennett Peter M. Department of Microbiology and Pathology, School of Medical Sciences, University of Bristol Bristol BS8 JTD, UK aut Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 423-431 0305-7453 37:3<423 1996 37 jac https://doi.org/10.1093/jac/37.3.423 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jac/37.3.423 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Walsh Timothy R. Department of Microbiology and Pathology, School of Medical Sciences, University of Bristol Bristol BS8 JTD, UK aut NATIONALLICENCE 700 1- Gamblin Steven National Medical Research Centre The Ridgeway, Millhill, London NW7 1AA, UK aut NATIONALLICENCE 700 1- Emery David C. National Medical Research Centre The Ridgeway, Millhill, London NW7 1AA, UK aut NATIONALLICENCE 700 1- MacGowan Alasdair P. Regional Antimicrobial Reference Laboratory, Southmead Health Services NHS Trust, Bristol BS10 5NB, UK aut NATIONALLICENCE 700 1- Bennett Peter M. Department of Microbiology and Pathology, School of Medical Sciences, University of Bristol Bristol BS8 JTD, UK aut NATIONALLICENCE 773 0- Journal of Antimicrobial Chemotherapy Oxford University Press 37/3(1996-03), 423-431 0305-7453 37:3<423 1996 37 jac Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford