caa a22 4500 44529177X CHVBK 20180317142530.0 cr unu---uuuuu 170323e20101201xx s 000 0 eng 10.1007/s12588-010-0002-6 doi (NATIONALLICENCE)springer-10.1007/s12588-010-0002-6 Synthesis and photo-physical properties of polymeric soft materials and its application in FRET based DNA sensor [Elektronische Daten] [Rati Nayak, N. Pradhan, L. Sukla, B. Mishra] Water-soluble conjugated polyelectrolytes (CPs) an ideal advanced soft materials have attracted considerable attention as an optical platform in chemical and biological assays for nucleic acids, proteins, enzyme activities and physiological metal ions. From the viewpoint of detection sensitivity, CPs-based assays benefit from their light-harvesting properties via collective response of optical units along the polymer backbone. In the present investigation we have successfully synthesized a cationic conjugated polymer via Suzuki copolymerization and successive quaternization. In order to enhance its optical property for efficient biosensor materials, a polymer-surfactants micellar complex approach has been made. In water, the molar absorptivity and fluorescence quantum efficiency of cationic poly(fluorene-co-phenylene) (c-PFP) were substantially increased in the presence of nonionic surfactants. About 400% enhancement of the FRET signal was measured in c-PFP/ssDNA-Fl with Brij 30, relative to that without surfactants. The signal amplification was successfully modulated using different type of nonionic surfactants which perturb the complexation, fine-structure of the complex (i.e., donor-acceptor separation) and the resulting energy transfer process. Central Institute of Plastics Engineering & Technology, 2010 Conjugated polyelectrolytes nationallicence Donor-acceptor systems nationallicence FRET nationallicence Fluorescence nationallicence Surfactants nationallicence Nayak Rati Council of Scientific & Industrial Research (CSIR), Institute of Minerals and Materials Technology, 751013, Bhubaneswar, India aut Pradhan N. Council of Scientific & Industrial Research (CSIR), Institute of Minerals and Materials Technology, 751013, Bhubaneswar, India aut Sukla L. Council of Scientific & Industrial Research (CSIR), Institute of Minerals and Materials Technology, 751013, Bhubaneswar, India aut Mishra B. Council of Scientific & Industrial Research (CSIR), Institute of Minerals and Materials Technology, 751013, Bhubaneswar, India aut International Journal of Plastics Technology Springer-Verlag 14(2010-12-01), 1-6 0972-656X 14<1 2010 14 12588 https://doi.org/10.1007/s12588-010-0002-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s12588-010-0002-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nayak Rati Council of Scientific & Industrial Research (CSIR), Institute of Minerals and Materials Technology, 751013, Bhubaneswar, India aut NATIONALLICENCE 700 1- Pradhan N. Council of Scientific & Industrial Research (CSIR), Institute of Minerals and Materials Technology, 751013, Bhubaneswar, India aut NATIONALLICENCE 700 1- Sukla L. Council of Scientific & Industrial Research (CSIR), Institute of Minerals and Materials Technology, 751013, Bhubaneswar, India aut NATIONALLICENCE 700 1- Mishra B. Council of Scientific & Industrial Research (CSIR), Institute of Minerals and Materials Technology, 751013, Bhubaneswar, India aut NATIONALLICENCE 773 0- International Journal of Plastics Technology Springer-Verlag 14(2010-12-01), 1-6 0972-656X 14<1 2010 14 12588 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer