caa a22 4500 445291958 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9276-4 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9276-4 Derivation of the King's College London human embryonic stem cell lines [Elektronische Daten] [Emma Stephenson, Peter Braude] Since the derivation of the first human embryonic stem cell (hESC) line in 1998, there has been substantial interest in the potential of these cells for regenerative medicine and cell therapy and in the use of hESCs carrying clinically relevant genetic mutations as models for disease research and therapeutic target identification. There is still a need to improve derivation efficiency and further the understanding of the basic biology of these cells and to develop clinical grade culture systems with the aim of producing cell lines suitable for subsequent manipulation for therapy. The derivation of initial hESC lines at King's College London is discussed here, with focus on derivation methodology. Each of the derivations was distinctive. Although the stage and morphology of each blastocyst were generally similar in each attempt, the behaviour of the colonies was unpredictable; colony morphology and development was different with each attempt. Days 5, 6 and 7 blastocysts were used successfully, and the number of days until appearance of stem-like cells varied from 4 to 14d. Routine characterisation analyses were performed on three lines, all of which displayed appropriate marker expression and survived cryopreservation—thaw cycles. From the lines discussed, four are at various stages of the deposition process with the UKSCB, one is pending submission and two are unsuitable for banking. Continued open and transparent reporting of results and collaborations will maximise the efficiency of derivation and facilitate the development of standardised protocols for the derivation and early culture of hESC lines. The Society for In Vitro Biology, 2010 IVF nationallicence Blastocyst nationallicence Derivation nationallicence Preimplantation genetic diagnosis nationallicence Human embryonic stem cells nationallicence Stephenson Emma Department of Women's Health and Division of Reproduction and Endocrinology, King's College London, London, UK aut Braude Peter Department of Women's Health and Division of Reproduction and Endocrinology, King's College London, London, UK aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 178-185 1071-2690 46:3-4<178 2010 46 11626 https://doi.org/10.1007/s11626-010-9276-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9276-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stephenson Emma Department of Women's Health and Division of Reproduction and Endocrinology, King's College London, London, UK aut NATIONALLICENCE 700 1- Braude Peter Department of Women's Health and Division of Reproduction and Endocrinology, King's College London, London, UK aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 178-185 1071-2690 46:3-4<178 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer