caa a22 4500 445291982 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9307-1 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9307-1 Establishment and maintenance of three Chinese human embryonic stem cell lines [Elektronische Daten] [Di Zhou, Ge Lin, Chang-Qing Xie, Bo Xiong, Xiao-Ying Zhou, Xiao-Bing Qian, Yi Liao, Guang-Xiu Lu] To establish a potential resource for cell therapy and a developmental model for human diseases, we had isolated three Chinese human embryonic stem cell lines from the inner cell mass of human blastocysts in 2002. All the three cell lines were grown on mouse embryonic fibroblasts as feeder cells; one of these cell lines, chHES-3, has maintained its normal karyotype even after being cultured in vitro for more than 100 passages, after the standardization of mouse feeder preparation. Each hES cell line has been completely characterized. All the three cell lines expressed hES-specific markers and pluripotency-related genes. These cells maintained their normal karyotype during long-term culture and displayed a high telomerase activity. When differentiated in vivo and in vitro, the derivatives representing the three germ layers could be observed. Human leukocyte antigen, ABO blood type, and DNA fingerprinting were also performed to provide a unique identity to each cell line. By establishing these hES cell lines, we provide an appropriate in vitro model to study human development and regeneration. All the three cell lines can be obtained for research purposes by placing a request at our website at www.hescbank.cn. The Society for In Vitro Biology, 2010 Embryonic stem cell lines nationallicence Self-renewal nationallicence Pluripotency nationallicence Zhou Di Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut Lin Ge Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut Xie Chang-Qing Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut Xiong Bo Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut Zhou Xiao-Ying Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut Qian Xiao-Bing Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut Liao Yi Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut Lu Guang-Xiu Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 192-199 1071-2690 46:3-4<192 2010 46 11626 https://doi.org/10.1007/s11626-010-9307-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9307-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Di Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Lin Ge Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Xie Chang-Qing Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Xiong Bo Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Zhou Xiao-Ying Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Qian Xiao-Bing Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Liao Yi Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Lu Guang-Xiu Institute of Human Reproduction and Stem Cell Engineering, Central South University, National Center of Human Stem Cells Research and Engineering, No. 88 Xiang-Ya Road, 410078, Changsha, Hunan, China aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 192-199 1071-2690 46:3-4<192 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer