caa a22 4500 445291990 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9291-5 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9291-5 Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines [Elektronische Daten] [Maria Camarasa, Robbie Kerr, Sharon Sneddon, Nicola Bates, Lisa Shaw, Rachel Oldershaw, Fiona Small, Melissa Baxter, Tristan Mckay, Daniel Brison, Susan Kimber] We report here the derivation of two new human embryonic stem cell lines, Man-1 and Man-2, and their full characterization as novel pluripotent stem cell lines. Man-1 was derived from an embryo surplus to requirement from routine IVF, while Man-2 was obtained from an oocyte classified as failed to fertilise and subsequently chemically activated. We report the characterisation of pluripotency and the differentiation potential of these lines. Work is in progress to establish novel methods of stem cell derivation and culture, which will avoid the use of xenobiotics and be relevant to clinical production of human embryonic stem cell lines. Both newly derived human embryonic stem cell lines will be available for the research community from the UK Stem Cell Bank (http://www.ukstemcellbank.org.uk). The Society for In Vitro Biology, 2010 Stem cells nationallicence Derivation nationallicence Research grade nationallicence Camarasa Maria North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut Kerr Robbie North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut Sneddon Sharon North West Embryonic Stem Cell Centre, University of Manchester, Manchester Academic Health Science Centre, Central Manchester University Hospitals NHS Foundation Trust, St Mary's Hospital, M13 OJH, Manchester, UK aut Bates Nicola North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut Shaw Lisa North West Embryonic Stem Cell Centre, University of Manchester, Manchester Academic Health Science Centre, Central Manchester University Hospitals NHS Foundation Trust, St Mary's Hospital, M13 OJH, Manchester, UK aut Oldershaw Rachel North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut Small Fiona North West Regional Cytogenetics Unit, St Mary's Hospital, Manchester Royal Infirmary, Central Manchester University Hospitals NHS Foundation Trust, Oxford Road, M13 9WL, Manchester, UK aut Baxter Melissa North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut Mckay Tristan North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut Brison Daniel North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut Kimber Susan North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 386-394 1071-2690 46:3-4<386 2010 46 11626 https://doi.org/10.1007/s11626-010-9291-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9291-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Camarasa Maria North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut NATIONALLICENCE 700 1- Kerr Robbie North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut NATIONALLICENCE 700 1- Sneddon Sharon North West Embryonic Stem Cell Centre, University of Manchester, Manchester Academic Health Science Centre, Central Manchester University Hospitals NHS Foundation Trust, St Mary's Hospital, M13 OJH, Manchester, UK aut NATIONALLICENCE 700 1- Bates Nicola North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut NATIONALLICENCE 700 1- Shaw Lisa North West Embryonic Stem Cell Centre, University of Manchester, Manchester Academic Health Science Centre, Central Manchester University Hospitals NHS Foundation Trust, St Mary's Hospital, M13 OJH, Manchester, UK aut NATIONALLICENCE 700 1- Oldershaw Rachel North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut NATIONALLICENCE 700 1- Small Fiona North West Regional Cytogenetics Unit, St Mary's Hospital, Manchester Royal Infirmary, Central Manchester University Hospitals NHS Foundation Trust, Oxford Road, M13 9WL, Manchester, UK aut NATIONALLICENCE 700 1- Baxter Melissa North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut NATIONALLICENCE 700 1- Mckay Tristan North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut NATIONALLICENCE 700 1- Brison Daniel North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut NATIONALLICENCE 700 1- Kimber Susan North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, M13 3PT, Manchester, UK aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 386-394 1071-2690 46:3-4<386 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer