caa a22 4500 445292016 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9308-0 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9308-0 Derivation of 30 human embryonic stem cell lines—improving the quality [Elektronische Daten] [Susanne Ström, Frida Holm, Rosita Bergström, Anne-Marie Strömberg, Outi Hovatta] We have derived 30 human embryonic stem cell lines from supernumerary blastocysts in our laboratory. During the derivation process, we have studied new and safe method to establish good quality lines. All our human embryonic stem cell lines have been derived using human foreskin fibroblasts as feeder cells. The 26 more recent lines were derived in a medium containing serum replacement instead of fetal calf serum. Mechanical isolation of the inner cell mass using flexible metal needles was used in deriving the 10 latest lines. The lines are karyotypically normal, but culture adaptation in two lines has been observed. Our human embryonic stem cell lines are banked, and they are available for researchers. The Author(s), 2010 Human embryonic stem cells nationallicence Feeder cells nationallicence Human fibroblasts nationallicence Defined medium nationallicence Ström Susanne Department of Clinical Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden aut Holm Frida Department of Clinical Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden aut Bergström Rosita Department of Clinical Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden aut Strömberg Anne-Marie Department of Clinical Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden aut Hovatta Outi Department of Clinical Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 337-344 1071-2690 46:3-4<337 2010 46 11626 https://doi.org/10.1007/s11626-010-9308-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9308-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ström Susanne Department of Clinical Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden aut NATIONALLICENCE 700 1- Holm Frida Department of Clinical Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden aut NATIONALLICENCE 700 1- Bergström Rosita Department of Clinical Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden aut NATIONALLICENCE 700 1- Strömberg Anne-Marie Department of Clinical Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden aut NATIONALLICENCE 700 1- Hovatta Outi Department of Clinical Science, Technology and Intervention, Karolinska Institutet, Karolinska University Hospital Huddinge, SE 141 86, Stockholm, Sweden aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 337-344 1071-2690 46:3-4<337 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer