caa a22 4500 445292032 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9293-3 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9293-3 Derivation of new human embryonic stem cell lines from preimplantation genetic screening and diagnosis-analyzed embryos [Elektronische Daten] [Adeleh Taei, Hamid Gourabi, Ali Seifinejad, Mehdi Totonchi, Ebrahim Shahbazi, Mojtaba Valojerdi, Poopak Eftekhari, Leila Karimian, Hossein Baharvand] In this study, we focused on the derivation of human embryonic stem cell (hESC) from preimplantation genetic screening (PGS)-analyzed and preimplantation genetic diagnosis (PGD)-analyzed embryos. Out of 62 fresh PGD/PGS-analyzed embryos, 22 embryos reached the blastocyst stage. From 12 outgrowth blastocysts, we derived four hESC lines onto a feeder layer. Surprisingly, karyotype analysis showed that hESC lines derived from aneuploid embryos had diploid female karyotype. One hESC line was found to carry a balanced Robertsonian translocation. All the cell lines showed hESC markers and had the pluripotent ability to differentiate into derivatives of the three embryonic germ layers. The established lines had clonal propagation with 22-31% efficiency in the presence of ROCK inhibitor. These results further indicate that hESC lines can be derived from PGD/PGS-analyzed embryos that are destined to be discarded and can serve as an alternative source for normal euploid lines. The Society for In Vitro Biology, 2010 Human embryonic stem cells nationallicence Preimplantation genetic screening nationallicence Robertsonian translocation nationallicence Taei Adeleh Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box19395-4644, Tehran, Iran aut Gourabi Hamid Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut Seifinejad Ali Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box19395-4644, Tehran, Iran aut Totonchi Mehdi Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut Shahbazi Ebrahim Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box19395-4644, Tehran, Iran aut Valojerdi Mojtaba Department of Embryology, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut Eftekhari Poopak Department of Embryology, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut Karimian Leila Department of Embryology, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut Baharvand Hossein Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box19395-4644, Tehran, Iran aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 395-402 1071-2690 46:3-4<395 2010 46 11626 https://doi.org/10.1007/s11626-010-9293-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9293-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taei Adeleh Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box19395-4644, Tehran, Iran aut NATIONALLICENCE 700 1- Gourabi Hamid Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut NATIONALLICENCE 700 1- Seifinejad Ali Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box19395-4644, Tehran, Iran aut NATIONALLICENCE 700 1- Totonchi Mehdi Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut NATIONALLICENCE 700 1- Shahbazi Ebrahim Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box19395-4644, Tehran, Iran aut NATIONALLICENCE 700 1- Valojerdi Mojtaba Department of Embryology, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut NATIONALLICENCE 700 1- Eftekhari Poopak Department of Embryology, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut NATIONALLICENCE 700 1- Karimian Leila Department of Embryology, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut NATIONALLICENCE 700 1- Baharvand Hossein Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box19395-4644, Tehran, Iran aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 395-402 1071-2690 46:3-4<395 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer