caa a22 4500 445292059 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9299-x doi (NATIONALLICENCE)springer-10.1007/s11626-010-9299-x Establishment and characterization of human embryonic stem cell lines, Turkey perspectives [Elektronische Daten] [Zafer Candan, Semra Kahraman] Human embryonic stem cells (hESC), which are derived from the inner cell mass (ICM) of blastocyst stage embryos, are of great importance because of their unpredictable two unique features: their differentiation ability into all types of cells derived from three germ layers and their potentially unlimited capacity of self renewing with stable karyotype. These distinguished properties make hESC very promising cell source for regenerative medicine, tissue replacement therapies, and drug screening studies as well as genomics. However, due to the several technical problems, such as risk of teratoma formation, immune response, and unknown genetic pathways for lineage specific differentiation, and ethical drawbacks of their using in clinical treatments, hESC researches are still waiting to advance beyond to animal trials and drug studies. During the last decade, more than 300 new hESC lines have been derived and published by researchers worldwide. However, despite their similar well-known unique properties, recent studies reported that hESC lines have very individual properties and are differed from each other with regards to their differentiation ability and gene expression profiles. Therefore, all hESC lines should be characterized in detail and then registered in a stem cell bank for generating global database. In this report, the characteristic of hESC lines, which were established in Istanbul Memorial Hospital between 2003 and 2005, and derivation methods were described in detail to inform researchers and to facilitate new prospective cooperative studies. The Society for In Vitro Biology, 2010 Human embryonic stem cells nationallicence PGD nationallicence In vitro differentiation nationallicence Blastocysts nationallicence Candan Zafer Istanbul Memorial Hospital, ART & Reproductive Genetics Unit, Piyale Pasa Bulvari, Okmeydani, 34385, Istanbul, Turkey aut Kahraman Semra Istanbul Memorial Hospital, ART & Reproductive Genetics Unit, Piyale Pasa Bulvari, Okmeydani, 34385, Istanbul, Turkey aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 345-355 1071-2690 46:3-4<345 2010 46 11626 https://doi.org/10.1007/s11626-010-9299-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9299-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Candan Zafer Istanbul Memorial Hospital, ART & Reproductive Genetics Unit, Piyale Pasa Bulvari, Okmeydani, 34385, Istanbul, Turkey aut NATIONALLICENCE 700 1- Kahraman Semra Istanbul Memorial Hospital, ART & Reproductive Genetics Unit, Piyale Pasa Bulvari, Okmeydani, 34385, Istanbul, Turkey aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 345-355 1071-2690 46:3-4<345 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer