caa a22 4500 445292067 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9298-y doi (NATIONALLICENCE)springer-10.1007/s11626-010-9298-y Derivation of three new human embryonic stem cell lines [Elektronische Daten] [Cara Bradley, Omar Chami, Teija Peura, Alexis Bosman, Biljana Dumevska, Uli Schmidt, Tomas Stojanov] Human embryonic stem cells are pluripotent cells capable of extensive self-renewal and differentiation to all cells of the embryo proper. Here, we describe the derivation and characterization of three Sydney IVF human embryonic stem cell lines not already reported elsewhere, designated SIVF001, SIVF002, and SIVF014. The cell lines display typical compact colony morphology of embryonic stem cells, have stable growth rates over more than 40 passages and are cytogenetically normal. Furthermore, the cell lines express pluripotency markers including Nanog, Oct4, SSEA3 and Tra-1-81, and are capable of generating teratoma cells derived from each of the three germ layers in immunodeficient mice. These experiments show that the cell lines constitute pluripotent stem cell lines. The Society for In Vitro Biology, 2010 Derivation nationallicence Human nationallicence Embryonic stem cells nationallicence Pluripotent nationallicence Bradley Cara Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut Chami Omar Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut Peura Teija Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut Bosman Alexis Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut Dumevska Biljana Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut Schmidt Uli Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut Stojanov Tomas Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 294-299 1071-2690 46:3-4<294 2010 46 11626 https://doi.org/10.1007/s11626-010-9298-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9298-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bradley Cara Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Chami Omar Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Peura Teija Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Bosman Alexis Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Dumevska Biljana Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Schmidt Uli Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut NATIONALLICENCE 700 1- Stojanov Tomas Sydney IVF Stem Cells, 321 Kent St, 2000, Sydney, NSW, Australia aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 294-299 1071-2690 46:3-4<294 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer