caa a22 4500 445292075 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9303-5 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9303-5 Human embryonic stem cells from aneuploid blastocysts identified by pre-implantation genetic screening [Elektronische Daten] [Kavita Narwani, Juan-Carlos Biancotti, Tamar Golan-Lev, Nicole Buehler, David Hill, Sagiv Shifman, Nissim Benvenisty, Neta Lavon] Human embryonic stem cells are derived from the inner cell mass of pre-implantation embryos. The cells have unlimited proliferation potential and capacity to differentiate into the cells of the three germ layers. Human embryonic stem cells are used to study human embryogenesis and disease modeling and may in the future serve as cells for cell therapy and drug screening. Human embryonic stem cells are usually isolated from surplus normal frozen embryos and were suggested to be isolated from diseased embryos detected by pre-implantation genetic diagnosis. Here we report the isolation of 12 human embryonic stem cell lines and their thorough characterization. The lines were derived from embryos detected to have aneuploidy by pre-implantation genetic screening. Karyotype analysis of these cell lines showed that they are euploid, having 46 chromosomes. Our interpretation is that the euploid cells originated from mosaic embryos, and in vitro selection favored the euploid cells. The undifferentiated cells exhibited long-term proliferation and expressed markers typical for embryonic stem cells such as OCT4, NANOG, and TRA-1-60. The cells manifested pluripotent differentiation both in vivo and in vitro. To further characterize the different lines, we have analyzed their ethnic origin and the family relatedness among them. The above results led us to conclude that the aneuploid mosaic embryos that are destined to be discarded can serve as source for normal euploid human embryonic stem cell lines. These lines represent various ethnic groups; more lines are needed to represent all populations. The Author(s), 2010 Human embryonic stem cells nationallicence Derivation nationallicence Aneuploidy nationallicence Pre-implantation genetic screening nationallicence Cell repository nationallicence Narwani Kavita The International Stem Cell Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA aut Biancotti Juan-Carlos The International Stem Cell Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA aut Golan-Lev Tamar Department of Genetics, The Institute of Life Sciences, The Hebrew University, Jerusalem, Israel aut Buehler Nicole ART Reproductive Center, Beverly Hills, CA, USA aut Hill David ART Reproductive Center, Beverly Hills, CA, USA aut Shifman Sagiv Department of Genetics, The Institute of Life Sciences, The Hebrew University, Jerusalem, Israel aut Benvenisty Nissim The International Stem Cell Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA aut Lavon Neta The International Stem Cell Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 309-316 1071-2690 46:3-4<309 2010 46 11626 https://doi.org/10.1007/s11626-010-9303-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9303-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Narwani Kavita The International Stem Cell Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Biancotti Juan-Carlos The International Stem Cell Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Golan-Lev Tamar Department of Genetics, The Institute of Life Sciences, The Hebrew University, Jerusalem, Israel aut NATIONALLICENCE 700 1- Buehler Nicole ART Reproductive Center, Beverly Hills, CA, USA aut NATIONALLICENCE 700 1- Hill David ART Reproductive Center, Beverly Hills, CA, USA aut NATIONALLICENCE 700 1- Shifman Sagiv Department of Genetics, The Institute of Life Sciences, The Hebrew University, Jerusalem, Israel aut NATIONALLICENCE 700 1- Benvenisty Nissim The International Stem Cell Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Lavon Neta The International Stem Cell Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 309-316 1071-2690 46:3-4<309 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer