caa a22 4500 445292083 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9285-3 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9285-3 Derivation, characterization, differentiation, and registration of seven human embryonic stem cell lines (VAL-3, -4, -5, -6M, -7, -8, and -9) on human feeder [Elektronische Daten] [Cristobal Aguilar-Gallardo, Maria Poo, Eva Gomez, Amparo Galan, Eva Sanchez, Ana Marques-Mari, Veronica Ruiz, Jose Medrano, Marcia Riboldi, Diana Valbuena, Carlos Simon] Derivation of human embryonic stem cell lines has been a remarkable scientific achievement during the last decade. Human embryonic stem cells are regarded as an unlimited cell source for replacement therapy in regenerative medicine. Clearly, the scientific community requires proper derivation, characterization, and registration with the purpose of making them available for research and future medical applications worldwide. In this paper, we report our derivation work as the Valencian Node of the Spanish Stem Cell Bank in the generation, characterization, and registration of VAL-3, -4, -5, -6M, -7, -8, and 9 (www.isciii/htdocs/terapia/terapia_bancocelular.jsp). The derivation process was performed on microbiologically tested and irradiated human foreskin fibroblasts and designed to minimize contact with xeno-components in knockout Dulbecco's modified Eagle's medium supplemented with knockout serum replacement and basic fibroblast growth factor. Fingerprinting of the cell lines was performed to allow their identification and traceability. All lines were expressed at the mRNA and specific protein markers for undifferentiation and were found to be negative for classical differentiation markers such as neurofilament heavy chain (ectoderm), renin (mesoderm), and amylase (endoderm). All lines displayed high levels of telomerase activity and were shown to successfully overcome cryopreservation and thawing. Finally, we demonstrated the potential to differentiate in vitro (embryoid body formation) and in vivo (teratoma formation) into cell types from all three germ layers. Teratoma derived from all human embryonic stem cell lines present similar morphological features except VAL-8 that display more aggressive tumor behavior with a larger proportion of solid tissues, as opposed to cyst formation in the other cell lines. The Society for In Vitro Biology, 2010 hESC lines nationallicence Derivation nationallicence Xeno-free conditions nationallicence VAL-3 to VAL-9 nationallicence Aguilar-Gallardo Cristobal Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut Poo Maria Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut Gomez Eva Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut Galan Amparo Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut Sanchez Eva Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut Marques-Mari Ana Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut Ruiz Veronica Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut Medrano Jose Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut Riboldi Marcia Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut Valbuena Diana Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut Simon Carlos Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 317-326 1071-2690 46:3-4<317 2010 46 11626 https://doi.org/10.1007/s11626-010-9285-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9285-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aguilar-Gallardo Cristobal Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut NATIONALLICENCE 700 1- Poo Maria Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut NATIONALLICENCE 700 1- Gomez Eva Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut NATIONALLICENCE 700 1- Galan Amparo Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut NATIONALLICENCE 700 1- Sanchez Eva Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut NATIONALLICENCE 700 1- Marques-Mari Ana Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut NATIONALLICENCE 700 1- Ruiz Veronica Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut NATIONALLICENCE 700 1- Medrano Jose Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut NATIONALLICENCE 700 1- Riboldi Marcia Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut NATIONALLICENCE 700 1- Valbuena Diana Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut NATIONALLICENCE 700 1- Simon Carlos Valencian Node of the Spanish Stem Cell Bank, Prince Felipe Research Centre (CIPF), Valencia, Spain aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 317-326 1071-2690 46:3-4<317 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer