caa a22 4500 445292091 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9278-2 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9278-2 Derivation and maintenance of human embryonic stem cell line on human adult skin fibroblast feeder cells in serum replacement medium [Elektronische Daten] [R. Tecirlioglu, Linh Nguyen, Karen Koh, Alan Trounson, Anna Michalska] Human embryonic stem (hES) cells were originally isolated and maintained on mouse embryonic fibroblast (MEF) feeder layers in the presence of fetal bovine serum (FBS). However, if the hES cells are to be used for therapeutic applications, it is preferable to regulatory authorities that they be derived and cultured in animal-free conditions to prevent mouse antigen contamination that would exacerbate an immune response to foreign proteins, and the potential risk of transmission of retroviral and other zoonotic pathogens to humans. As a step towards this goal, we derived a new hES cell line (MISCES-01) on human adult skin fibroblasts as feeder cells using serum replacement (SR) medium. The MISCES-01 cells have a normal diploid karyotype (46XX), express markers of pluripotency (OCT4, GCTM-2, TRA-1-60, TRA-1-81, SSEA-3, SSEA-4, and alkaline phosphatase) and following in vitro and in vivo differentiation, give rise to derivatives of the three primary germ layers. This cell line can be obtained for research purposes from the Australian Stem Cell Centre (http://www.stemcellcentre.edu.au). The Society for In Vitro Biology, 2010 Human embryonic stem cells nationallicence Serum replacement medium nationallicence Human feeders nationallicence Tecirlioglu R. Research Office, Monash University, 3800, Clayton, VIC, Australia aut Nguyen Linh Monash Immunology and Stem Cell Laboratories (MISCL), Monash Science Technology, Research and Innovation Precinct (STRIP), Monash University, 3800, Clayton, VIC, Australia aut Koh Karen Monash Immunology and Stem Cell Laboratories (MISCL), Monash Science Technology, Research and Innovation Precinct (STRIP), Monash University, 3800, Clayton, VIC, Australia aut Trounson Alan California Institute for Regenerative Medicine, 210 King Street, 94107, San Francisco, CA, USA aut Michalska Anna Monash Immunology and Stem Cell Laboratories (MISCL), Monash Science Technology, Research and Innovation Precinct (STRIP), Monash University, 3800, Clayton, VIC, Australia aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 231-235 1071-2690 46:3-4<231 2010 46 11626 https://doi.org/10.1007/s11626-010-9278-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9278-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tecirlioglu R. Research Office, Monash University, 3800, Clayton, VIC, Australia aut NATIONALLICENCE 700 1- Nguyen Linh Monash Immunology and Stem Cell Laboratories (MISCL), Monash Science Technology, Research and Innovation Precinct (STRIP), Monash University, 3800, Clayton, VIC, Australia aut NATIONALLICENCE 700 1- Koh Karen Monash Immunology and Stem Cell Laboratories (MISCL), Monash Science Technology, Research and Innovation Precinct (STRIP), Monash University, 3800, Clayton, VIC, Australia aut NATIONALLICENCE 700 1- Trounson Alan California Institute for Regenerative Medicine, 210 King Street, 94107, San Francisco, CA, USA aut NATIONALLICENCE 700 1- Michalska Anna Monash Immunology and Stem Cell Laboratories (MISCL), Monash Science Technology, Research and Innovation Precinct (STRIP), Monash University, 3800, Clayton, VIC, Australia aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 231-235 1071-2690 46:3-4<231 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer