caa a22 4500 445292105 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9286-2 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9286-2 Skottman Heli Regea-Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, Biokatu 12, 33520, Tampere, Finland aut Derivation and characterization of three new human embryonic stem cell lines in Finland [Elektronische Daten] [Heli Skottman] Human embryonic stem cell (hESC) lines can be established from the preimplantation embryos. Due to their ability to differentiate into all three embryonic layers, hESC are of significant interest as a renewable source of cell material for different applications, especially for cell replacement therapy. Since the establishment of the first hESC lines in 1998, several studies have described the derivation and culture of new hESC lines using various derivation methods and culture conditions. Our group has currently established eight new hESC lines of which three of the latest ones are described in a more detailed way in this report. The described lines have been established using mechanical derivation methods for surplus bad quality embryos and culture conditions containing human foreskin fibroblast feeder cells and serum-free culture medium. All the new lines have a normal karyotype and typical hESC characteristics analyzed in vitro. The described hESC lines are available for research purposes upon request (www.regea.fi). The Society for In Vitro Biology, 2010 Human embryonic stem cell nationallicence Derivation nationallicence Culture nationallicence Characterization nationallicence In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 206-209 1071-2690 46:3-4<206 2010 46 11626 https://doi.org/10.1007/s11626-010-9286-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9286-2 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Skottman Heli Regea-Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital, Biokatu 12, 33520, Tampere, Finland aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 206-209 1071-2690 46:3-4<206 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer