caa a22 4500 445292113 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9281-7 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9281-7 Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark [Elektronische Daten] [Linda Harkness, Iben Rasmussen, Karin Erb, Moustapha Kassem] The derivation and characterisation of human embryonic stem cells provides a source of pluripotent stem cells with potential for clinical applications. Utilising locally sourced embryos from two IVF clinics, we derived and characterised five new cell lines for use in a non-clinical setting. Analysis of clinical data showed that the majority of embryos (94.5%) failed to reach the blastocyst stage of development and of all embryos, regardless of developmental status, 248 embryos were needed to create one stem cell line. From the number of embryos (69) which developed to the blastocyst stage 8.7% developed into cell lines. Using outgrowth of the whole blastocyst, we derived five new, unreported cell lines in Odense, Denmark between 2005 and 2006. Characterisation was carried out using RT-PCR, staining, karyotyping, EB formation and teratoma formation. The KMEB hESC lines will, in the future, be made available through the UK Stem Cell Bank (http://www.ukstemcellbank.org.uk/). The Society for In Vitro Biology, 2010 Human embryonic stem cells nationallicence Derivation nationallicence Characterisation nationallicence Harkness Linda Molecular Endocrinology laboratory (KMEB), Medical Biotechnology Centre, University of South Denmark, Winslowparken 25, 1 sal, 5000, Odense, Denmark aut Rasmussen Iben Fertility Clinic, Odense University Hospital, Odense, Denmark aut Erb Karin Fertility Clinic, Odense University Hospital, Odense, Denmark aut Kassem Moustapha Molecular Endocrinology laboratory (KMEB), Medical Biotechnology Centre, University of South Denmark, Winslowparken 25, 1 sal, 5000, Odense, Denmark aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 259-268 1071-2690 46:3-4<259 2010 46 11626 https://doi.org/10.1007/s11626-010-9281-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9281-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harkness Linda Molecular Endocrinology laboratory (KMEB), Medical Biotechnology Centre, University of South Denmark, Winslowparken 25, 1 sal, 5000, Odense, Denmark aut NATIONALLICENCE 700 1- Rasmussen Iben Fertility Clinic, Odense University Hospital, Odense, Denmark aut NATIONALLICENCE 700 1- Erb Karin Fertility Clinic, Odense University Hospital, Odense, Denmark aut NATIONALLICENCE 700 1- Kassem Moustapha Molecular Endocrinology laboratory (KMEB), Medical Biotechnology Centre, University of South Denmark, Winslowparken 25, 1 sal, 5000, Odense, Denmark aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 259-268 1071-2690 46:3-4<259 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer