caa a22 4500 445292172 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9277-3 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9277-3 Analysis of long-term culture properties and pluripotent character of two sibling human embryonic stem cell lines derived from discarded embryos [Elektronische Daten] [Parvathy Venu, Sanjukta Chakraborty, Maneesha Inamdar] We had earlier reported the derivation and characterization of two new sibling human embryonic stem cell lines BJNhem19 and BJNhem20, from discarded grade III embryos of Indian origin. We report here the characteristics of the two sibling cell lines after long-term continuous culture for over 2yr during which they have been passaged over 200 times. We show that both cell lines adapt well to culture on various mouse and human feeders as well as in feeder-free conditions. The cells show normal diploid karyotype and continue to express all pluripotency markers. Both cell lines differentiate to derivatives of all three germ layers in vitro. However as reported earlier, BJNhem19 is unable to generate teratomas in nude or SCID mice or differentiate to beating cardiomyocytes when tested over several passages during long-term stable culture. On the other hand, the cardiac differentiation capacity of BJNhem20 is greatly increased, and it can generate beating cardiomyocytes that proliferate when isolated and cultured further. In conclusion, the two cell lines have maintained a stable phenotype for over 2yr and are indeed immortal. Their derivation from grade III embryos does not seem to have any adverse effect on their long-term phenotype. The cells can be obtained for research purposes from the UK Stem Cell Bank and from the authors. The Author(s), 2010 BJNhem19 nationallicence BJNhem20 nationallicence Sibling nationallicence Human embryonic stem cell nationallicence Long-term culture nationallicence Venu Parvathy Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., 560064, Bangalore, India aut Chakraborty Sanjukta Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., 560064, Bangalore, India aut Inamdar Maneesha Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., 560064, Bangalore, India aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 200-205 1071-2690 46:3-4<200 2010 46 11626 https://doi.org/10.1007/s11626-010-9277-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9277-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Venu Parvathy Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., 560064, Bangalore, India aut NATIONALLICENCE 700 1- Chakraborty Sanjukta Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., 560064, Bangalore, India aut NATIONALLICENCE 700 1- Inamdar Maneesha Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., 560064, Bangalore, India aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 200-205 1071-2690 46:3-4<200 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer