caa a22 4500 445292180 CHVBK 20180317142531.0 cr unu---uuuuu 170323e20100401xx s 000 0 eng 10.1007/s11626-010-9282-6 doi (NATIONALLICENCE)springer-10.1007/s11626-010-9282-6 Human embryonic stem cell lines isolation, cultivation, and characterization [Elektronische Daten] [Maria Lagarkova, Artem Eremeev, Anatoly Svetlakov, Nikolay Rubtsov, Sergei Kiselev] A large number of human embryonic stem cell (hESC) lines have been derived worldwide since the first hESC line establishment in 1998. Despite many common characteristics, most important of which is the pluripotency, hESC lines vary significantly in their transcriptional profiles, genetic, and epigenetic state. These differences may arise both from individual genetics of the cell lines and from variations in their handling such as isolation and cultivation. In order to minimize the latter differences, the standardized protocols of cultivation and inter-laboratory comprehensive studies should be performed. In this report, we summarized our experience of derivation and characterization of hESC lines as well as of adaptation of hESCs to novel cultivation protocols. We have successfully derived five hESC lines and characterized them by previously established criteria, including expression of specific markers and the capacity to differentiate both in vitro and in vivo. Four of these lines, namely hESM01-04, were initially derived using mouse fibroblasts as a feeder and currently are maintained under feeder-free, serum-free conditions using mTeSR1 and Matrigel. The fifth line, hESMK05 was derived in feeder-free, serum-free conditions using mTeSR1 and Matrigel. Cell lines retain their pluripotent status and normal karyotype for more than 70 passages and are available to the scientific community. The Society for In Vitro Biology, 2010 hESC lines nationallicence Isolation nationallicence Characterization nationallicence Lagarkova Maria Vavilov Institute of General Genetics RAS, Gubkina str., 3, 119991, Moscow, Russia aut Eremeev Artem Center for Reproductive Medicine, Krasnoyarsk, Russia aut Svetlakov Anatoly Center for Reproductive Medicine, Krasnoyarsk, Russia aut Rubtsov Nikolay Institute of Cytology and Genetics RAS, Novosibirsk, Russia aut Kiselev Sergei Vavilov Institute of General Genetics RAS, Gubkina str., 3, 119991, Moscow, Russia aut In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 284-293 1071-2690 46:3-4<284 2010 46 11626 https://doi.org/10.1007/s11626-010-9282-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11626-010-9282-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lagarkova Maria Vavilov Institute of General Genetics RAS, Gubkina str., 3, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Eremeev Artem Center for Reproductive Medicine, Krasnoyarsk, Russia aut NATIONALLICENCE 700 1- Svetlakov Anatoly Center for Reproductive Medicine, Krasnoyarsk, Russia aut NATIONALLICENCE 700 1- Rubtsov Nikolay Institute of Cytology and Genetics RAS, Novosibirsk, Russia aut NATIONALLICENCE 700 1- Kiselev Sergei Vavilov Institute of General Genetics RAS, Gubkina str., 3, 119991, Moscow, Russia aut NATIONALLICENCE 773 0- In Vitro Cellular & Developmental Biology - Animal Springer-Verlag 46/3-4(2010-04-01), 284-293 1071-2690 46:3-4<284 2010 46 11626 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer